DNA can't directly order a cell to make proteins. It has to be transcribed into RNA orribonucleic acid. RNA, in turn, is translated bycellular machineryto make amino acids, which it joins together to form polypeptides and proteins...
RNA、蛋白质之间的传递有三种一般情况（DNA 到 DNA，DNA 到 RNA，RNA 到蛋白质，可以发生在大多数细胞...
Themessenger RNAthen moves out of thenucleusinto thecytoplasm. Proteinfactoriesin thecytoplasm, calledribosomes, bind to themessenger RNA. Theribosomereads thecodein themessenger RNAto produce a chain made up ofamino acids. There are 20 different types ofamino acid. Transfer RNAmolecules carry the...
2.7.U4 Transcription is the synthesis of mRNA copied from the DNA base sequences by RNA polymerase.2.7.U6 The amino acid sequence of polypeptides is determined by mRNA according to the genetic code 2.7.U7 Codons of three bases on mRNA correspond to one amino acid in a polype...
考虑到含有pDNA的裂解物中存在的杂质,因此必须从细胞碎片和其他杂质如RNA、蛋白质和基因组DNA(genomic DNA, gDNA)中完全分离pDNA。这些杂质中大多数与pDNA具有一些相似的物理化学性质,如负电荷(RNA、gDNA和内毒素)、分子质量(gDNA和内毒素)和疏水性(内毒素),这极大地限制了分离过程[2]。
The assay described here can be more generally applied to detect other topoisomerases complexes, as well as other types of DNA-protein crosslinks. Briefly, cells are lysed using a solution containing a chaotropic salt, which allows for rapid extraction of nucleic acids (DNA and RNA). DNA is ...
J. Ku protein targeting by Ku70 small interfering RNA enhances human cancer cell response to topoisomerase II inhibitor and gamma radiation. Mol. Cancer Ther. 4, 529–536 (2005). CAS PubMed Google Scholar Malik, M. & Nitiss, J. L. DNA repair functions that control sensitivity to ...
Platinum compounds can interact with RNA [169,170] or various proteins which may confer the platinum-based compound’s side effects and toxicity [171,172]. Although physically different, cisplatin and oxaliplatin produce similar adducts at analogous locations in the DNA molecule [173]. The ...
The other DNA strand is called the coding strand, because its sequence is the same as the RNA sequence that is produced, with the exception of U replacing T. Since the RNA sequence is the sequence that we use to determine what amino acids are produced through mRNA, this DNA strand is al...
Initiation:Transcription begins at a promoter: a specific region of a gene. RNA polymerase binds to the promoter. This signals the DNA to unwind. The enzyme is now ready to make mRNA Elongation:Nucleotides are added to the mRNA strand