DNA and Aspects of Molecular Biology Reference work 1999,Comprehensive Natural Products Chemistry John D.Pickert,Benjamin L.Miller Explore book 7.18.4.1Choosing a Source of Template for PCR Although thePCRcan be used to amplify and detect DNA from miniscule samples (hence its popularity in forensic...
Journal2004, Current Opinion in Chemical Biology Christopher T Calderone, David R Liu Researchers have used the first approach to perform up to three reactions on a single DNA template (Figure 4a). For example, Gartner, Liu and co-workers used a DNA template displaying a primary amine group ...
Each of the two strands of the DNA molecule acts as a template for the synthesis of a complementary strand. Related Subjects DNA synthesis Fragile sites Origin firing Origin selection Replisome Stalled forks Translesion synthesis Latest Research and Reviews Mechanism for local attenuation of...
Genome Biology 13, R87 (2012). Article PubMed PubMed Central Google Scholar Stefansson, O. A. et al. A DNA methylation-based definition of biologically distinct breast cancer subtypes. Molecular Oncology (2014). Liu, H. et al. DNA methylation dynamics: identification and functional annotation...
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摘要:PolⅣ和PolV作为E.coli中的Y家族DNA聚合酶,分别由dinB和umuDC基因编码。由于其特殊结构,PolⅣ和PolV可对带有非编码损伤的DNA进行移损合成。移损合成作为一种损伤耐受机制,可在DNA损伤暂时未被修复的情况下保持DNA复制的连续性和基因组的完整性,但保真度相较同作为损伤耐受机制的依赖于同源重组的后复制缺口修...
(10 mM), 0.375 μL of Taq polymerase, and 1 μL of template DNA. The PCR conditions consisted of initial denaturation at 94 °C for 5 min; followed by 30 cycles of denaturation at 94 °C for 30 s, annealing at 53 °C for 30 s, extension at 72 °C for 1 min; a final ...
DNA synthesis is a complicated process involving anabolic processes to create the purine and pyrimidine nucleotide triphosphates required for replication, unwinding of the template DNA to provide access to the replication machinery, and the high fidelity process of creating complementary strands. ...
A modified SELEX (Systematic Evolution of Ligands by Exponential Enrichment) pr,otocol (referred to as PT SELEX) was used to select primer–template (P/T) sequences that bound to the vaccinia virus polymerase catalytic subunit (E9) with enhanced affinity. A single selected P/T sequence (refer...
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