【1】DNA or deoxyribonucleic acid is the molecule that codes genetic information. However, DNA can't directly order a cell to make proteins. It has to be transcribed into RNA orribonucleic acid. RNA, in turn, is translated bycell...
We demonstrate that base pairing of the incoming nucleoside with the protruding DNA end serves as a template for the incorporation and governs the yield of the elongated product. The efficiency of the template-directed process has been exploited by using BfiI for the site-specific modification of...
This short stretch of RNA serves as a template for telomere extension and plays a major role in leading strand extension; when DNA replication is completed, telomerase binds to the 3′-end of the leading strand. This establishes base pairing with the short stretch of RNA sequence the ...
Figure 17.1.MEGAWHOP is based on the replacement of a homologous region between a fragment and vector. MEGWAHOP allows for the use of various types of fragments as long as they can hybridize to a template vector. In MEGAWHOP, the DNA fragment to be cloned serves as a set of complementary...
When DSBs occur, the MRE11/RAD50/NBS1 (MRN) complex binds to DSBs rapidly and functions as a DSBs sensor, which further recruits the protein kinase ataxia-telangiectasia mutated (ATM) and triggers ATM activation. ATM-mediated H2A.X phosphorylation at S139 serves as a platform for scaffold ...
1). However, the success in building large DNA circuits is mainly attributed to the principle of toehold-mediated strand displacement, the modular construction approach, and digital logic. During signal processing, because of the toehold that serves as a region for an incoming strand to attach ...
question came from the double-stranded nature of the structure: because each strand of DNA contains a sequence of nucleotides that is exactly complementary to the nucleotide sequence of its partner strand, each strand can act as a template, or mold, for the synthesis of a new complementary ...
All members of the PD-(D/E)XK superfamily share a common structural core, comprising a mixed β-sheet of 4 strands flanked on both sides by α-helices [1, 2, 13](Figure 1). It serves as a scaffold for a weakly conserved active site, typically including two or three acidic residues ...
Thus, the quantification of integrated DNA, which is the most stable form of HIV-DNA and serves as the main template for gene transcription and viral replication and is important for viral latency is thought to be the best indicator of the HIV reservoir size. However, it presents several ...
Actually as a minichromosome, cccDNA then serves as template for RNA polymerase II-mediated synthesis of the viral transcripts. All are 5 capped and 3 polyadenylated; their distinct start sites are defined by four separate promoters (see Figure 1A) but all use the same polyadenylation signal. ...