The DNAtemplateis enclosed by thenuclear envelope, which is fenestrated bynuclear pore complexes(NPCs).NPCsare large protein assemblies that transport key molecules in and out of the nucleus and consist of approximately 30 different subunits, termednucleoporins(Nups). Mutations inNupshave been linked...
例如,识别细菌复制区的蛋白质是DnaA蛋白,识别真核生物核DNA复制起始区的是起始区识别蛋白质复合体(Orc1～Orc6),识别古菌复制区的蛋白质是Orc1/Cdc6。不同真核生物的复制起始区通常可以互用,例如人的复制起始区在酵母中也能起作用。但是,一种细菌的复制起始区只有在亲缘关系十分密切的其他细菌中才能互换。
RNA can act as a template for DNA synthesis in the reverse transcription of retroviruses and retrotransposons and in the elongation of telomeres. Despite its abundance in the nucleus, there has been no evidence for a direct role of RNA as a template in the repair of any chromosomal DNA lesion...
DNA is one of the most popular building blocks of nanotechnology and is commonly used to construct ordered nanoscale structures with controlled architectures. For the most part, DNA is looked upon as a promising building block for fabricating microelectronic circuits from the bottom up. Now a team ...
Wickens, How the messenger got its tail: addition of poly(A) in the nucleus, Biochemical Sciences,1990 vol. 15 splicing (剪切) During the processing, some sequences are cut out from the central regions and the outer portions joined. These intervening sequences, or introns , interrupt those ...
What is a sequence transcribed from DNA that remains in the RNA after RNA processing called? This also refers to the segments of DNA from which this sequence was transcribed. What type of RNA carries instructions from DNA in the nucleus to the ribosome in the cytoplasm?
Genomic DNAis organized three-dimensionally in the nucleus as chromatin. Recent accumulating evidence has demonstrated that chromatin organizes into numerous dynamic domains in higher eukaryotic cells, which act as functional units of the genome. These compacted domains facilitateDNA replicationand gene regu...
We engineered a tagged version of the yeast Rad51 recombinase and used this tool to monitor DNA double-strand break repair in living cells. We could observe how a broken DNA fragment can scout the nucleus to identify a similar sequence and use it as a template for repair. ...
The jumbo phage ΦKZ constructs a proteinaceous nucleus-like compartment around its genome that protects phage DNA from degradation by DNA-targeting immune effectors of the host, including CRISPR–Cas and restriction enzymes. Senén D. Mendoza , Eliza S. Nieweglowska & Joseph Bondy-Denomy Article...
And particularly their selective binding to DNA through π-electron core of DNA effects by a complex way the duplex DNA. It is well-known that genomic DNA is densely packed inside the cell nucleus and viral capsids. Such close packing suggests that electrostatic repulsion between negatively ...