James GS. Universal bacterial identification by PCR and DNA sequencing of 16S rRNA gene. Schuller M, Sloots TP, James GS, Halliday CL, Carter IW, eds., PCR for Clinical Microbiology: An Australian and International Perspective. Dordrecht NLD: Springer Science and Business Media B.V.; 2010...
PCR for Clinical Microbiology Volume 876 || Universal Bacterial Identification by PCR and DNA Sequencing of 16S rRNA Gene M Schuller,TP Sloots,GS James,... 被引量: 0发表: 2010年 PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found ...
Joseph, M., Wong, E. Y., ... & Yuen, K. Y. (2008). Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. Clinical microbiology and infection, 14(10), 908-934.这些...
在本研究中,我们开发了一种Cas-16S-seq方法来减轻16S rRNA基因扩增子测序中的宿主污染。这种方法使用Cas9和特异性gRNA特异性切割宿主16S rRNA基因,从而在16S-seq扩增子文库制备过程中富集细菌序列(图1),以水稻为例,我们建立了一个生物信息学流程来设计特异性的gRNA,使其能够从数百万个原核16S rRNA基因中区分出水稻...
Universal Bacterial Identification by PCR and DNA Sequencing of 16S rRNA Gene The assay is a single amplification PCR incorporating three separate reactions. The primers used are broad range primers which recognise conserved sequences within the 16S rRNA gene and amplify the... G James - Springer...
aRNA (rRNA) gene sequence (16S) and taxonomic profiles, whole-genome shotgun (WGS) or metagenomic sequencing of whole community DNA, and alignment of the assembled sequences to the reference RNA (rRNA)基因序列(16S)和分类学外形,完全染色体猎枪(WGS)或metagenomic被装配的序列的程序化整体社区脱氧核糖...
We investigated whether the DNA extraction methods and modifications affect the diversity and composition of microbial communities identified through high-throughput sequencing of 16S rRNA gene. Results DNA yields Both modified phenol–chloroform methods yielded a higher phenol-contaminated DNA but a lower ...
The limitation of 16S rRNA gene sequencing (DNA-based) for microbial community analyses in water is the inability to differentiate live (dormant cells as well as growing or non-growing metabolically active cells) and dead cells, which can lead to false positive results in the absence of live ...
Through introduction of Next-generation Sequencing (NGS), the 16S rRNA method is now also widely used to deconvolute complex microbial communities. NGS is the best technique to study any microbiome as Sanger sequencing fails to detect multiple bacteria in one sample and culture is labor inten...
mitochondrial 16S rRNA gene fulfills the requirements for a universal DNA barcoding marker;we also show certain advantage in the application and universality of combination.Furthermore,our result supports the opinion:"We strongly advocate the use of 16S rRNA as standard DNA barcoding marker for ...