Woo, P.C., Lau, S.K., Teng, J.L., Tse, H., Yuen, K.Y. Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. Clin Microbiol Infect. 14 (10):908-934. (2008) Tang, Y.W., Ellis, N.M.,...
本研究中,作者利用Sakinaw湖中8个不同深度的湖水样本基于PacBio平台对全长16S rDNA进行测序,同时也利用Miseq 平台对16S rDNA的V4区域测序。结果表明,V4数据中有0.2-4.1%的微生物在门水平上无法区分,而PhyloTags则能在门水平上完全区分。作者认为在环境样本中,短读长测序数据在复杂群类区分中失去优势,而长读长则...
细菌16S rDNA基因 宏基因组测序则是将微生物基因组DNA随机打断成500bp的小片段,然后在片段两端加入通用引物进行PCR扩增测序,再通过组装的方式,将小片段拼接成较长的序列。 研究目的不同 16S测序主要研究群落的物种组成、物种间的进化关系以及群落的多样性。 宏基因组测序在16S测序分析的基础上还可以进行基因和功能层面...
(The 16S rDNA V4 region was amplified by PCR and sequenced in the MiSeq platform (Illumina) using the 2×250 bp paired-end protocol yielding pair-end reads that overlap almost completely. The primers used for amplification contain adapters for MiSeq sequencing and single-index barcodes so tha...
Microbiota analysis with next-generation 16S rDNA gene sequencing in recurrent common bile duct stones[1]. 研究方案:内镜下逆行胰胆管造影术(ERCP)是胆结石的主要治疗方法,但术后复发率很高。最近研究表明胆道微生物群参与胆石症的发病机制。然而胆道微生物群失调与复发性胆石症相关性机制尚未得到研究;本课题通...
天津军事科学院军事医学研究院环境医学与作业医学研究所王静教授团队在Frontiers in Cellular and Infection Microbiology上发文“Integrative Analysis of Vaginal Microorganisms and Serum Metabolomics in Rats With Estrous Cycle Disorder Induced by LongTerm Heat Exposure Based on 16S rDNA Gene Sequencing and LC/MS-...
· · Woo, P. C., Lau, S. K., Teng, J. L., Tsang, A. K., Joseph, M., Wong, E. Y., ... & Yuen, K. Y. (2008). Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. Clinical micro...
· · Woo, P. C., Lau, S. K., Teng, J. L., Tsang, A. K., Joseph, M., Wong, E. Y., ... & Yuen, K. Y. (2008). Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. Clinical micro...
·· Woo, P. C., Lau, S. K., Teng, J. L., Tsang, A. K., Joseph, M., Wong, E. Y., ... & Yuen, K. Y. (2008). Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. Clinical microbi...
Matsuo, Y., Komiya, S., Yasumizu, Y. et al. Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution. BMC Microbiol 21, 35 (2021)。 PacBio 16S全长测序:一种高效且经济的微生物组研究方法。