16S rRNAMetagenomicSimulatorSparseCount dataMicrobiomeCompositionalIn the last few years, 16S rRNA gene sequencing (16S rDNA-seq) has seen a surprisingly rapid increase in election rate as a methodology to perform microbial community studies. Despite the considerable popularity of this technique, an ...
1. Johnson J S , Spakowicz D J , Hong B Y , et al. Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis[J]. Nature Communications, 2019, 10(1). 2. Pootakham Wirulda,Mhuantong Wuttichai,Yoocha Thippawan et al. High resolution profiling of coral-as...
(Characterizing human fecal microbial communities Methods for V4-16S rRNA gene sequencing and data analysis, calculation of MAZ scores and functional microbiome maturity, and quantification of enteropathogen burden by means of multiplex quantitative polymerase chain reaction (qPCR) are described in the sup...
宏基因组测序(Metagenomics Sequencing)是对环境样品中全部微生物的总DNA进行高通量测序,主要研究微生物种群结构、基因功能、微生物之间的相互协作关系以及微生物与环境之间的关系。宏基因组测序研究摆脱了微生物分离纯培养的限制,扩展了微生物资源的利用空间,为环境微生物群落的研究提供了有效工具。 高通量测序平台 16S扩...
In this study, this method was employed to predict the potential functions of each sample based on 16S rRNA sequencing data. We used the KEGG database[和/或 COG database等] and performed closed reference OTU picking using the sampled reads against a Greengenes reference taxonomy (Greengenes ...
The 16S rRNA gene amplicon sequence data from tailing and nontailing rhizosphere soils of Mimosa pudica from a heavy metal-contaminated area are reported here. Diverse bacterial taxa were represented in the results, and the most dominant phyla were Proteobacteria (41.2%), Acidobacteria (17.1%), ...
The next steps involve the construction of the sequencing library and the sequencing workflow, with 16S rRNA sequencing generally employing short read lengths to cover specific areas of the 16S rRNA gene. The final phase involves quality control and filtering of the sequencing data, followed by util...
与基因表达、代谢分子、蛋白等数据一起分析共表达网路或者共表达模式(co-occurrence and co-exclusion patterns)。 参考文献:JuF, ZhangT. 16s rRNA gene high throughput sequencing data mining of microbiota diversity and interactions, Appl Microbiol Biotechnol. 2015, 99(10):4119-4129...
16S rRNA 测序:一种基于 PCR 的细菌物种鉴定技术16S rRNA Sequencing: A PCR-based Technique to Identify Bacterial Species 概述 资料来源:Ewa Bukowska-Faniband1、Tilde Andersson1、Rolf Lood1 1 隆德临床科学系,感染医学科,生物医学中心,隆德大学,221 00 隆德,瑞典 ...
流程的安装程序与测序数据可从网址(http://www.ligene.cn/win16s/ )免费下载使用。关键词:16SrRNA;微生物组;Linux;QIIME;VSEARCH中图分类号:Q752 文献标志码:A 文章编号:1672-5565(2018)04-239-07Asimplepipelinetoanalyze16SrRNAampliconsequencingdataintheWindowsFANGMeimei,WANGYuxuan,WANGMingyue,ZHENGHelong,...