sequencing and variant calling alongside high-accuracy epigenetic base calling. The platform comprises an all-enzyme workflow with extremely high conversion efficiencies, thus enabling accurate data from valuable, low-input biological samples or from clinical cfDNA. The readout of genetic or epigenetic ...
Next-generation sequencing (NGS) has transformed genomic research by decreasing the cost of sequencing. However, whole-genome sequencing is still costly and complex for diagnostics purposes. In the clinical space, targeted sequencing has the advantage of
To this end, we performed bulk RNA-sequencing (RNA-seq) of total RNA isolated from individual mouse hippocampi obtained either 96 h or 21 days after contextual fear conditioning (CFC) (one-trial 3 min exposure to a context followed by a 2 s, 0.7 mA shock, constant current), ...
60°C primer annealing Direct gel loading High fidelity Platinum SuperFi II DNA Polymerase preserves DNA sequence accuracy with its extremely low error rate. Usingnext-generation sequencing, the relativefidelityof Platinum SuperFi II DNA polymerase was calculated to be >300x that ofTaq...
Here, we analyzed nucleosome positioning in the asexual and sexual stages of the parasite’s erythrocytic cycle using chromatin immunoprecipitation of MNase-digested chromatin, followed by next-generation sequencing. We observed a relatively open chromatin structure at the trophozoite and gametocyte stage...
Sequencing analysis further demonstrated the generation of eccDNA harboring targeted TSG mutations in these tumor cells. Conclusions Small eccDNAs carrying TSG mutations may serve as an important source supporting intratumor heterogeneity and tumor evolution in mouse liver cancer induced by multiplexed CRISPR...
Technical notes Improved protocol for SARS-CoV-2 genome sequencing with PCR enrichment Fidelity measurement of Phusion Plus DNA Polymerase Type IIs gene assembly using PCR-generated inserts and vectors Successful amplification of AT-rich DNA sequences with Phusion Plus DNA Polym...
method based on fountain code (LT code) and achieved the DNA storage and completely accurate recovery for a 2.15 MB data file (including text, operating system, image, PDF, movie, and malware) using lower sequencing depth (average coverage: ~ 10.5 ×) (Erlich and Zielinski 2017). ...
Blood TCRA T cell fraction is higher in females than in males and correlates with both tumour immune infiltrate and presence of bacterial sequencing reads. Tumour TCRA T cell fraction is prognostic in lung adenocarcinoma. Using a meta-analysis of tumours treated with immunotherapy, we show that ...
Although biologically derived protein nanopores are ideally suited for DNA sequencing6, solid-state nanopores offer control over pore size during the fabrication process, allowing for the detection of a wide range of analytes. Although this versatility permits the analysis of a variety of biomolecules,...