Methods – sequencing First-Generation Sequencing The capillary electrophoresis analyzer, better known as a “DNA sequencer,” can be used for both traditional Sanger DNA sequencingandfor detection of insertions/deletions by sizing of PCR products (“fragment size analysis”), offering single base resol...
Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease. This second edition of...
NucTools: analysis of chromatin feature occupancy profiles from high-throughput sequencing data. BMC Genomics 18, 158 (2017). Article PubMed PubMed Central Google Scholar Voong, L. N. et al. Insights into nucleosome organization in mouse embryonic stem. Cells Chem. Mapp. Cell 167, 1555–...
Blood TCRA T cell fraction is higher in females than in males and correlates with both tumour immune infiltrate and presence of bacterial sequencing reads. Tumour TCRA T cell fraction is prognostic in lung adenocarcinoma. Using a meta-analysis of tumours treated with immunotherapy, we show that ...
(LNP-M) demonstrated a stable nanoparticle structure, high expression efficiency, and low toxicity. Notably, a DNA vaccine encoding the spike protein, delivered via LNP-M, induced stronger antigen-specific antibody and T cell immune responses compared to electroporation. Single-cell RNA sequencing (...
Technical notes Improved protocol for SARS-CoV-2 genome sequencing with PCR enrichment Fidelity measurement of Phusion Plus DNA Polymerase Type IIs gene assembly using PCR-generated inserts and vectors Successful amplification of AT-rich DNA sequences with Phusion Plus DNA Polymera...
Circle-Seq: Isolation and sequencing of chromosome-derived circular DNA elements in cells. Methods Mol Biol. 2020;2119:165–81. PubMed Google Scholar Prada-Luengo I, Krogh A, Maretty L, Regenberg B. Sensitive detection of circular DNAs at single-nucleotide resolution using guided realignment...
To this end, we performed bulk RNA-sequencing (RNA-seq) of total RNA isolated from individual mouse hippocampi obtained either 96 h or 21 days after contextual fear conditioning (CFC) (one-trial 3 min exposure to a context followed by a 2 s, 0.7 mA shock, constant current), ...
DNA comprises molecular information stored in genetic and epigenetic bases, both of which are vital to our understanding of biology. Most DNA sequencing approaches address either genetics or epigenetics and thus capture incomplete information. Methods widely used to detect epigenetic DNA bases fail to ...
The most commonly used targeted sequencing methods are based on Sequencing by Synthesis (SBS), including those from Ion Torrent and Illumina. Ion Torrent’s SBS method detects the byproduct of natural nucleotide incorporation, H+, by ISFET sensors7. The usage of natural nucleotides results in sev...