Determination of the precise order of nucleotides within a DNA molecule is popularly known as DNA sequencing. About three decades ago in the year 1977, Sanger and Maxam–Gilbert made a breakthrough that revolutionized the world of biological sciences by sequencing the 5,386-base bacteriophage φ...
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近日,杂志Nature chemistry上发表了一篇题为“Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics”的综述文章。在这篇综述中,作者描述了纳米孔DNA测序的生化创新,并讨论了基于纳米孔的肽测序的技术现状并探索...
1. DNA sequencing methods Common DNA sequencing methods include whole-genome sequencing,de novosequencing, targeted sequencing, and exome sequencing (discussed below) (Figure 2). DNA may also be sequenced for epigenetic studies—e.g...
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Programs have been written to apply parallel processing algorithms to the main methods of DNA sequence analysis. These programs allow the largest of currently interesting problems to be handled on a medium-sized computer system. The abundance of information otherwise not readily available has suggested...
Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics Adam Dorey Stefan Howorka Nature Chemistry(2024) Single-molecule RNA sizing enables quantitative analysis of alternative transcription termination Gerardo Patiño-Guillén ...
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sequencing, automation and sample preparation (sequence up to 384 DNA samples in a single batch run in up to 24 runs a day), large sequencing strategies (directly sequence only relative short, 300–1000 nucleotides, longDNA fragmentsin a single reaction) andnew sequencing methods(high-throughput...