crosslinks which can inhibit transcription and replication in addition to prompting DNA breaks38; we then tested whether the NTD or CTD variants rendered cells sensitive to other forms of replication stress. We exposed cells to hydroxyurea (HU), a drug that reduces the pool of dNTPs leading to ...
Using eccDNA production as a readout, further genetic screens identified factors from alt-EJ as essential for retrotransposon replication. alt-EJ drives the second-strand synthesis of the long terminal repeat retrotransposon DNA through a circularization process and is therefore necessary for eccDNA ...
the components that make DNA, dropped below the critical level needed for replication,” noted Oxford’s Andy Ryan. “Starved of these building blocks, the cells die. Importantly, normal cells in the body do not have SETD2 mutations, so these effects of WEE1 inhibition are potentially...
To update your Cisco IMC firmware, first see the release notes for the corresponding release of Cisco DNA Center that you are installing. In the release notes, the “Supported Firmware” section shows the Cisco IMC firmware version for your Cisco ...
There were large multifocal nuclear accumulations of γH2AX and 53BP1 in wild-type and Ifnar1fl/fl mice injected with Syn-cre, as previously found in nuclear bodies formed around DNA lesions in cell lines undergoing replication stress28, with an average size of 2 µm corresponding to ...
Characterization of an episome produced in hamster cells that amplify a transfected CAD gene at high frequency: functional evidence for a mammalian replication origin. Mol Cell Biol. 1987;7:1740–50. CAS PubMed PubMed Central Google Scholar Ly P, Brunner SF, Shoshani O, Kim DH, Lan W, ...
DNA double-strand breaks (DSBs) can be caused by environmental agents such as radiation and drugs1,2,3and endogenous metabolism such as transcription and replication stress4,5. For example, normal metabolism has been estimated to cause ~1–50 DSBs per human cell per day6,7. Consequently, fas...
To update your Cisco IMC firmware, first see the release notes for the corresponding release of Cisco DNA Center that you are installing. In the release notes, the “Supported Firmware” section shows the Cisco IMC firmware version for your Cisco DNA...
(CCAN) is shown to enable precise reloading of centromere-bound CENP-A onto the same DNA sequences as in its initial prereplication loading. Thus, DNA replication acts as an error correction mechanism for maintaining centromere identity through its removal of non-centromeric CENP-A coupled with ...
samples from 59 tissue types, originating from 185 mammalian species across 19 taxonomic orders (Supplementary Data1.1–1.4and Supplementary Notes1and2) with ages ranging from prenatal to 139 years old (bowhead whale,Balaena mysticetus)9. These data are a subset from our Mammalian Methylation ...