“When WEE1 was inhibited in cells with a SETD2 mutation, the levels of deoxynucleotides, the components that make DNA, dropped below the critical level needed for replication,” noted Oxford’s Andy Ryan. “Starved of these building blocks, the cells die. Importantly, normal cells in the ...
DNA replication and segregation are essential in all life forms. In bacteria, partitioning (Par) systems are responsible for the efficient segregation of replicated genetic material (chromosome, low-copy-number plasmids) to daughter cells1,2,3,4. Theparloci are divided into three different types,...
Chromatin is the template for the basic processes of replication and transcription, making the maintenance of chromosomal integrity critical for cell viability. To elucidate how dividing cells respond to alterations in chromatin structure, here we analyse the replication programme of primary cells with al...
Replication stress (RS) is a major source of genomic instability and is intrinsic to cancer cells. RS is also the consequence of chemotherapeutic drugs for treating cancer. However, adaptation to RS is also a mechanism of resistance to chemotherapy. BRCA2 deficiency results in replication stress i...
There were large multifocal nuclear accumulations of γH2AX and 53BP1 in wild-type and Ifnar1fl/fl mice injected with Syn-cre, as previously found in nuclear bodies formed around DNA lesions in cell lines undergoing replication stress28, with an average size of 2 µm corresponding to ...
Characterization of an episome produced in hamster cells that amplify a transfected CAD gene at high frequency: functional evidence for a mammalian replication origin. Mol Cell Biol. 1987;7:1740–50. CAS PubMed PubMed Central Google Scholar Ly P, Brunner SF, Shoshani O, Kim DH, Lan W, ...
But on the "Configure standby" flow, the Configure replication step doesn't complete, leaving the Recovery site in the "Configuring Standby" state indefinitely. CSCwb17117 After a DR Pause + Backup and Restore + DR Rejoin workflow, the Con...
The target region was the short P6-loop of the chloroplast trnL (UAA) intron, amplified with the universal plant primers g and h (Taberlet et al., 2007). These primers amplify vascular plant taxa, but may also sporadically detect other non-vascular plants (Taberlet et al., 2018). ...
Supplementary Tables 1-5 listing activity data and short DNA and RNA sequences; Supplementary Sequences 1-3 that contain DNA and protein sequences of key constructs; Supplementary Notes 1-4 that contain FACS information, cloning methods, and scripts; Supplementary Figure 1 that contains original ge...
The DNA templates (T960, T1200, L1, L2, L3, L4 and L5) and bricks were designed by a computer-assisted method. First, the reported software40was used to generate a series of DNA brick sequences. By using the design software, a set of short DNA bricks with a length of 24 nt was...