. 2× native gel-loading buffer (per 100 mL) was made with 20 g sucrose, 10 mL of 10× TBE, 1 mL of 10% (w/v) SDS (sodium dodecyl sulphate), 25 mg bromophenol blue, and 25 mg xylene cyanol FF. 2× denaturing gel-loading buffer was made with the same recipe, ...
•在做质粒的上有没有这个酶切位点鉴定的时候，用原质粒和经过单酶切的质粒在同一块胶上进行电泳，看单酶切后的质粒是不是比原质粒跑得快从而鉴定出质粒有没有被切开。Recipe6xGel-loadingBufferI0.25%(w/v)bromophenolblue0.25%(w/v)xylenecyanolFF40%(w/v)sucroseinH2O DNASizeMigrationwithSample...
However, in the preferred embodiment of the bulk leaf maceration method, the following recipe is used: 0.1% w/v Tween-80; 0.04 M Tris-Cl, pH 7.7; 0.15 M NaCl; 0.1% w/v BSA-Pentex fraction V; 0.01% w/v sodium azide; 200 mM EDTA. The color dye tartrazin may optionally be added...
1 肾衰方活性成分的筛选结果 Figure 1 Wayne diagram of Shenshuai Recipe for the treatment of myocardial injury in chronic kidney disease 表 1 肾衰方中 Degree 值排名前十的化合物及 OB%,DL 值 Table 1 Top ten compounds with degree values and OB%, DL values in Shenshuai Recipe 活性成分 Active ...
1 肾衰方活性成分的筛选结果 Figure 1 Wayne diagram of Shenshuai Recipe for the treatment of myocardial injury in chronic kidney disease 表 1 肾衰方中 Degree 值排名前十的化合物及 OB%,DL 值 Table 1 Top ten compounds with degree values and OB%, DL values in Shenshuai Recipe 活性成分 Active ...
The phosphorylation of ssDNA was performed using the following recipe: 5 pmole of the single-stranded DNA pool, 20 units of T4 Polynucleotide Kinase (T4 PNK), 1 µL of 10× T4 ligase buffer and 1 µL of 10× T4 PNK buffer were mixed in a 10 µL total volume reaction. ...
The kit is shipped on dry ice. The DNAs should be stored at 4° C and the buffers stored at -20° C upon receipt. Avoid frequent freeze/thaw cycles with the plasmid as this may contribute to DNA breakage. Store enzyme at -70° C. We also recommend that the enzyme be ...
2. Prepare Lysozyme Digestion Buffer (see recipe on page 15). To ~200 μL Lysozyme Digestion Buffer/sample, add fresh Lysozyme to obtain a final Lysozyme concentration of 20 mg/mL. 3. Harvest up to 2 × 109 Gram positive cells by centrifugation. If you are using a frozen cell pellet,...
Cast a 5% non-denaturing polyacrylamide gel following the suggested recipe below. We have successfully used the Bio-Rad mini-PROTEAN casting system for these purposes. Dilute the Rgg protein to the desired concentration(s) in a solution ensuring its solubility (e.g. storage or binding buffer)....
place 0.2- 0.5 g (dry wt) lyophilized pad in a 5o ml disposable centrifuge tube, break up the pad wtih a spatula or glass rod, add c. 5 ml 3mm glass beads and powder the pad by brief shaking. add 10 ml (for a 0.5 g pad) of CTAB extraction buffer(see recipe, below), gently...