This application provides a detailed description of a washing solution consisting of saline sodium citrate (SSC) buffer and sodium dodecyl sulfate (SDS) for hybridization-enrichment-capture DNA sequencing libraries as well as a corresponding washing method. The invented solution has a simple recipe, ...
质粒DNA的大量提取和纯化实验——碱法 在制作酶谱、测定序列、制备探针等实验中需要高纯度、高浓度的质粒DNA,为此需要大量提取质粒DNA。大量提取的质粒DNA一般需进一步纯化,常用柱层析法和氯化绝梯度离心法。实验材料细菌试剂、试剂盒STE酚 氯仿NaClPEG乙醇仪器、耗材离心管离心机抽干机实验步骤1、取培养至对数生长后期...
9. Add 10μl 4M ammonium acetate and 1mL 100% EtOH. 10. Spin for 1 min in a microfuge, and discard the supernatant. Wash with 1ml 70% EtOH. Air-dry the pellet and resuspend in 50μl TE. Yeast Lysis Buffer (Winston Lysis Buffer) 200ml Triton X-100 4ml 10% SDS 20ml 5M NaCl ...
Component PureLink® Genomic Lysis/Binding Buffer PureLink® Genomic Digestion Buffer PureLink® Genomic Wash Buffer 1 PureLink® Genomic Wash Buffer 2 PureLink® Genomic Elution Buffer (10 mM Tris-HCl, pH 9.0, 0.1 mM EDTA) RNase A (20 mg/mL) in 50 mM Tris-HCl, pH 8.0, 10 mM ...
buffer B in the kit. Beads were resuspended in 50 µl of 10 mM Tris–HCl (pH 7) and incubated at 70 °C for 3 min for washing and then eluted with 10 µl of buffer. Eluate was then used for the second round of polyA enrichment in the same procedure as described ...
Fish DNA Barcoding Curriculum Manual 1 Kit Inventory Checklist Kit Components (included)* Resuspension solution, 5 ml Lysis solution, 5 ml Neutralization solution, 5 ml Matrix, 5 ml Wash buffer, 10 ml Spin columns Master mix for PCR, 1.2 ml Fish primer mix, 50 µl UView™ 6x loading ...
Flow-through was discarded and 50 µL M-Desulphonation Buffer was added to each well. The plates were incubated for 15 min at room temperature. Afterwards, they were spun at 2200 xG for 8 min, discarding the flow-through. 200 µL M-Wash Buffer was added to each well and spun...
The fact that DNA will grow old, is definitely a crap!!! DNA can be well-preserved in appropriate buffer solution at -20oC for years. Nowadays, DNA extraction kits are widely used in molecular biology laboratory. Since they had several old blood samples from Anwar, I believe they have lot...
The plugs were then washed with 0.5× TAE buffer three times with 5 min incubations. The plugs were loaded into a 1% certified low-melting-point agarose gel (Bio-Rad, 1613112) in 0.5× TAE buffer with ladders (CHEF DNA Size Marker, 0.2–2.2 Mb; Saccharomyces cerevisiae ladder, ...
Additionally, devices and methods of the invention employ wash solutions to reduce particle aggregation and remove unwanted sample, non-specific target entities, and buffer. Exemplary solutions include heparin, Tris-HCl, Tris-borate-EDTA (TBE), Tris-acetate-EDTA (TAE), Tris-cacodylate, HEPES (4-...