In step 2 of this method, "Muller Extraction Buffer" is used. The potential concentration ranges of the ingredients of the Muller Extraction Buffer are as follows: 0-2.0 % w/v Tween-80 0-2.0 M Tris-Cl, pH 6-8 0-2.0 M NaCl ...
1.Prepare CTAB Buffer (recipe attached). Prior to starting extraction, add polyvinylpyrrolidone and-mercaptoethanol. Once these have been added the shelf life of the buffer is only 2-3 days. CTAB Buffer PVP -mercaptoethanol 0.5 ml 0.02 g 2.5 uL 5 ml 0.2 g 25 uL 20 ml 0.8 g 100 uL Do...
add 10 ml (for a 0.5 g pad) of CTAB extraction buffer(see recipe, below), gently mix to wet all the powdered pad place in 65 C water bath for 30 min cool, add equal vol. ChCl3:IAA (24:1) mix, centrifuge in table top fuge 10 min at full speed transfer aqueous supernatant to a...
2.add 10 ml (for a 0.5 g pad) of CTAB extraction buffer(see recipe, below), gently mix to wet all the powdered pad 3.place in 65 C water bath for 30 min 4.cool, add equal vol. ChCl3:IAA (24:1) 5.mix, centrifuge in table top fuge 10 min at full speed 6.transfer aqueous ...
·L–1 PVP-40T (add before using) 2 g 2% (w/v) dH2O 84 mL –表3 缓冲液B配方(100 mL, 加dH2O定容) Table 3 Buffer B recipe (for 100 mL, bring the volume to 100 mL by adding dH2O) Stock Amount Final concentra- 1.0 mol·L–1Tris-HCl (pH8.0) 0.5 mol·L–1EDTA-Na2 5.0 mol...
In the laboratory, DNA extraction blanks (elution buffer from extraction kit) and PCR blanks were included. Data analysis Cq and melting temperature (Tm) values were used to determine the presence/absence of C. limbatus eDNA in a sample, by comparison with those of the standard curves and ...
在制作酶谱、测定序列、制备探针等实验中需要高纯度、高浓度的质粒DNA,为此需要大量提取质粒DNA。大量提取的质粒DNA一般需进一步纯化,常用柱层析法和氯化绝梯度离心法。实验材料细菌试剂、试剂盒STE酚 氯仿NaClPEG乙醇仪器、耗材离心管离心机抽干机实验步骤1、取培养至对数生长后期的含pBS质粒的细菌培养液250 ml,4 ℃...
The spin column-based cfDNA extraction was performed following the recommended protocol. The reagents in the QIAamp Circulating Nucleic Acid Kit (55114; QIAamp) were used to isolate cfDNA. Lysate buffer (ACL) containing 1 μg of carrier RNA was prepared prior to the experiment. The volume ...
7. Repeat ethanol extraction (Steps 5–6) once more. 8. Incubate the tubes with lid open at 37ºC for 5–10 minutes to evaporate any residual ethanol. 9. Add 180 μL PureLink® Genomic Digestion Buffer and 20 μL Proteinase K (supplied with the kit). Mix well by brief vortexing...
The phosphorylation of ssDNA was performed using the following recipe: 5 pmole of the single-stranded DNA pool, 20 units of T4 Polynucleotide Kinase (T4 PNK), 1 µL of 10× T4 ligase buffer and 1 µL of 10× T4 PNK buffer were mixed in a 10 µL total volume reaction. ...