DNA aptamer functionalized hydrogels for interferometric fiber-optic based continuous monitoring of potassium ions. Biosensors-Basel. 2021;11(8):266. Article CAS PubMed PubMed Central Google Scholar Han Y, Wu Y, Wang F, Li G, Wang J, Wu X, Deng A, Ren X, Wang X, Gao J, et al. ...
such as nascentDNA strandassay and 2D gel analysis, are also importantin vivoapproaches. Important quantitative parameters of replication, such as origin densities in the genome and rates of replication fork progression, are assessed using DNA fiber-based approaches where chromosomes are pulse-labeled ...
Functional nanomaterials with fluorescent or quenching abilities are important for the development of molecular probes for detection and studies of nucleic acids. Here, we describe a new class of molecular nanoprobes, the NanoCeracQ that uses nanoceria p
DNA fiber analysis Cells were pulse labeled with 25 μM CldU followed by 250 μM IdU for 20–45 min each. Labeled cells were trypsinized, lysed in spreading buffer (200 mM Tris-Hcl pH 7.4, 50 mM EDTA, and 0.5% SDS) and spread on microscope slide (Menzel-Gläser, Supe...
A triplex model based on fiber diffraction data with poly[d(T· A· T)] shows that the third pyrimidine strand is base paired according to the Hoogsteen pairing with an underlying A-type Watson–Crick duplex along the major groove (62, 63). The third strand is “parallel” to the ...
Principle of rcDNAzyme LAMP In this study, a 6-primer system (F3, B3, FIP, BIP, loop F and loop B) was used for LAMP reactions. LAMP primers were selected for the amplification of the E. coli phoA gene based on a previous work21. The primer sequences are shown in Table 1. All...
If a can‐ cer is composed of cells deficient in DNA repair, it is, in principle, vulnerable to agents that cause DNA damage. Thus a chemotherapeutic DNA damaging agent can be targeted to can‐ cers that lack the capability to repair the particular type of DNA damage caused by the agent...
7.6-fold longer circulation time in the blood compared to that of 1-NPs. The fiber-to-particle transformation can be performed in vivo after accumulation at the tumor site. The 1-NPs showed high photothermal conversion efficiency, good thermal stability, deep tissue penetration, and effective ...
638 nm 195 mW). The excitation beam was delivered to the optical bench via a single-mode polarisation-maintaining fiber (kineFLEX-HPV-P-3-S-405..640-0.7-0.7-P0, Qioptiq). The laser light was re-collimated after the fiber using an achromatic doublet lens (f = 50 mm) and direct...
The DNA Fragmentation Assay allows to determine the amount of DNA that is degraded upon treatment of cells with certain agents, e.g. with TNF-alpha or anti-Fas antibody (IPO-4). The principle of this assay is: the cells' DNA is radioactively labeled by growing the cells in presence of...