Totipotency emerges in early embryogenesis, but its molecular underpinnings remain poorly characterized. In the present study, we employed DNA fiber analysis to investigate how pluripotent stem cells are reprogrammed into totipotent-like 2-cell-like cell
DNA fiber assay was performed using an adapted method as described previously59. Briefly, 25 μM IdU (Sigma-Aldrich, I7125) was added to asynchronously growing cells, and incubated for 20 min at 37 °C. After washing twice with PBS, cells were incubated with CldU (250 μM, Sigm...
DNA meniscus in hollow fiber microinjection needle. The DNA flow rate can be readily monitored when using a hollow fiber injection needle. The meniscus can be used to determine or follow DNA flow during microinjection procedures. 2 Preparing Eggs for Microinjection Once the DNA is readied in ...
The repair products of double-stranded DNA breaks (DSBs) are crucial for investigating the mechanism underlying DNA damage repair as well as evaluating the safety and efficiency of gene-editing; however, a comprehensively quantitative assay remains to be established. Here, we describe the step-by-s...
15, Pages 9666-9680: Optical Fiber Nanotips Coated with Molecular Beacons for DNA Detection Optical fiber sensors, thanks to their compactness, fast response and real-time measurements, have a large impact in the fields of life science research, drug discovery and medical diagnostics. In recent ...
It seems that the mechanism of the interactive hepatoprotective impact of AE during the treatment with Cd might result mainly from the ability of the extract ingredients (first of all polyphenols, but also pectin and fiber) to chelate Cd2+ and in this way influence the body turnover of this ...
After an initial analysis of the entire tendon (100× magnification), the region with the greatest alteration in cell morphology was identified and characterized using the following characteristics: cell morphology, collagen fiber arrangement, the accumulation of ground substance, cellularity, and ...
英文名称:Fructan Assay Kit 型号规格:100次 品牌:爱尔兰Megazyme公司 果聚糖广泛分布于植物界。单子叶植物,双子叶植物和绿藻中均存在。 以分子结构和分子重量区别果聚糖。也可以分为三类:菊粉组,果聚糖组和带支链组。菊粉组主要或专门由果糖基果糖键组成。果聚糖组主要或专门由果糖基果糖键组成。带支链组由大量的...
To validate the microarray data, the expression of selected genes (AOX1,DHRS2,HIST1H2AC,ICAM4,MAP2K6, andOSMR) was quantified by real time RT-PCR. Genes that were found to be up- or downregulated by CDV in the microarray data were confirmed by RT-PCR assay (Figure2A) while those th...
DNA fiber assay, this method allows the analysis of single DNA molecules aligned on a slide; however, in this case, the DNA stretching is performed at a constant speed46. We found that ~50% of forks were able to restart upon release from HU in BRCA2 WT cells similar to previous ...