However, DNA isolation from plant cells surrounded by rigid polysaccharide cell walls involves complicated steps and requires benchtop laboratory equipment. As a result, plant DNA extraction is currently confined to well〆quipped laboratories and sample preparation has become one of the major hurdles for...
关键词:花生;基因组DNA;提取方法;幼叶 中图分类号:S565.201 文献标识号:A 文章编号:1001-4942(2014)07-0011-04 OptimizationofRapidDNAExtractionMethodsfromPeanutLeaves WangLei 1 ,ZhaoXintao 1 ,XuMengqi 2 ,SuFeng 1 ,RenYan 2 , LiShuangling 2 ,ShiYanmao 2 ,YuanMei 2倡 ,WangChuantang 2 (1.Chemic...
Conventional pre-genomics breeding methodologies have significantly improved crop yields since the mid-twentieth century. Genomics provides breeders with advanced tools for whole-genome study, enabling a direct genotype–phenotype analysis. This shift ha
Chloroplast isolation and chloroplast DNA extraction Leaves of 21-day-old plants were harvested to extract intact chloroplasts using a chloroplast isolation kit (Invent, CP-011) following the instructions from the manufacturer. Briefly, plant leaves were added to a filter column containing 200 µ...
Nuclei were isolated from the root cells and evaluated for the extent of DNA migration. With the exception of cycloheximide, all agents induced a significant increase in DNA migration. These results indicate that the comet assay may be a valuable tool for monitoring DNA damage in plant systems....
Specifically, gDNA was extracted from maize ear (1–3 cm), tassel (1 cm), and leaf (immature leaves after reproductive transition) using phenol:chloroform:IAA extraction. Five micrograms of genomic DNA was diluted in EB (10 mM Tris-HCl, pH 8.5) and sonicated to 200 bp fragments...
a diploid plant species, each cell has two copies of each nuclear genome (gDNA). As the plant ages the number of genomes per cell can increase; the penultimate stage of rosette leaves has an average of 13 genome copies per cell [2]. The number of mitochondria and plastids can vary in ...
Her current work focuses on the distribution and assembly mechanisms of microorganisms in soils, oceans, plant leaves, and insects.Geneticist and bioinformatician, Eric Coissac obtained his PhD in genetics and genomics in 1996 at the Université Pierre et Marie Curie in Paris. He is currently ...
英文名称:PureLink Genomic Plant DNA Purification Kit 产品类别: DNA提取与纯化 描述 The PureLink® Genomic Plant DNA Purification Kit allows efficient isolation of total DNA in about 40 minutes from a variety of plant samples, such as spinach, tomato, soy, wheat, Arabidopsis, and mushroom. •...
The experiment was performed in three bio- logical replicates. DNase I-chip. We established DNase I hypo-sensitivity profiling based on a published method52. Crude nuclei extracts were produced by treating 100 mg of ground frozen plant material in modified nuclei extraction buffer (20 mM PIPES-...