These methods are very fast, require little tissue and amplify well with plants that are amenable to DNA extraction and that contain few interfering secondary metabolites. However, the resulting DNA templates are not very pure and may not be stable for long periods of time. Recently, DNA purity...
This paper describes rapid and efficient DNA extraction methods for mature leaves, resting buds and seedling leaves of genera in the family Proteaceae. The procedures combine and modify previously published techniques. The DNA can be digested by restriction endonucleases and is suitable for subsequent ...
for extraction of DNA and RNA in this paper was simple,convenient and effective,it could be used to extract DNA and RNA not only from fungi,but also from plants,the DNA and RNA extracted using this method were stable and their activities were kept for a relatively long time during ...
Genomic DNA Purification Protocol from Plants Using Plant DNAzol Reagent Plasmid and cosmid purification protocols Plasmid and cosmid DNA extraction protocols are specialized methods for isolating circular DNA molecules from bacterial cells or other host organisms. Plasmids...
Genomic tools for plant breeding Traditional plant breeding involves selection and crossing of plants with desirable traits over several generations. Techniques include selection of superior individuals, hybridization, and backcrossing. Breeders aim to enhance traits like yield, disease resistance, and qualit...
We offer a range of Invitrogen genomic DNA extraction kits for sensitive, scalable purification from an expansive set of starting materials to help maximize process efficiency and downstream performance. This includes a ...
For the climatic conditions of this region, there is great diversity in plants, animals and microorganisms. In the Papaloapan region different fruits are grown, however, the main crops are sugarcane and pineapple. In this work the extraction of DNA from soils of sugarcane cultivation was performed...
For instance, metal ions such as iron, zinc and tin can easily bind to template DNA through the formation of direct crosslink and inhibit PCR by blocking access of polymerase to the DNA11,12,13. In addition, the co-extraction of DNA from solid and liquid samples rich in chemicals and ...
Part of the book series:Techniques in Life Science and Biomedicine for the Non-Expert((TLSBNE)) Abstract Unlike the animal cell, DNA extraction from plant cells faces challenges of a rigid cellulose cell wall and the presence of a variety of cytoplasmic components like polysaccharides, polyphenols...
analysis of SSR data, non-gel techniques for plant genotyping, and other applications of DNA profiling. As indicated by the editor, this book is intended for researchers in plant genetics, molecular biology, breeding, and biotechnology. In summary,Plant Genotyping: The DNA Fingerprinting of Plants...