Incubates which include candidate agents which alter transcription factor binding deviate from control incubates in terms of label signal - typically, binding is disrupted and the signal is diminished. In a preferred embodiment, the entire process is performed by a computer-controllable electromechanical ...
Although the proteins that read the gene regulatory code, transcription factors (TFs), have been largely identified, it is not well known which sequences TFs can recognize. We have analyzed the sequence-specific binding of human TFs using high-throughput SELEX and ChIP sequencing. A total of 83...
We have identified previously two transcription factors, COUP (chicken ovalbumin upstream promoter) and S300-II, from HeLa cell nuclear extracts. In this paper, the purine base and the phosphate backbone contact sites for the COUP transcription factor were defined. These studies indicate that the CO...
Hepatocyte nuclear factor 4 (HNF-4) is an orphan intracellular receptor that appears to be a key factor in the regulation of energy metabolism. In order to gain greater understanding of the binding and activation requirements of HNF-4, we performed genetic analysis of the apoCIII promoter, a ...
aligand to the DNA-binding domain of the p53 transcription factor ligand到p53副本因素的脱氧核糖核酸束缚的领域 [translate] 英语翻译 日语翻译 韩语翻译 德语翻译 法语翻译 俄语翻译 阿拉伯语翻译 西班牙语翻译 葡萄牙语翻译 意大利语翻译 荷兰语翻译 瑞典语翻译 希腊语翻译 51La ...
Binding transcription factor to DNA Double-strand DNA (NF-kB target sequence) was immobilized on a sensor, and the reaction of transcription factor (p50) and the signal amplification (sensitization) by antibody were observed.1.Protocol The measurement was made with the following conditions: NAPiCO...
a我们应将主要以力集中在周围 We should mainly concentrate by the strength in periphery[translate] acollection of monomeric units, and bond-forming processes[translate] aligand to the DNA-binding domain of the p53 transcription factor.[translate]...
and somehow standard in vitro methods can be used for determining the DNA-binding specificity of a particular transcription factor (see below). These have been recently backed up by a high-throughput heterologous method, the bacterial one-hybrid (B1H) system, whose potential advantages and pitfalls...