DNA-binding proteins are proteins that bind to single- or double-stranded DNA, generally in the major groove if the binding is sequence-specific – as with transcription factors that regulate expression of genes, and nucleases that cleave DNA between nucleotides. DNA-binding proteins can also bind...
近日来自日本名古屋大学的中野英男等人在Biotechnology and Bioengineering上发表的《Increasing cell‐free gene expression yields from linear templates in Escherichia coli and Vibrio natriegens extracts by using DNA‐binding proteins》开发了一种名为CroP-LET的方法,它通过dsDNA与单链Cro蛋白末端的结合来阻断线性...
The structures of three proteins that regulate gene expression have been determined recently and suggest how these proteins may bind to their specific recognition sites on the DNA. One protein (Cro) is a repressor of gene expression, the second (CAP) usually stimulates gene expression, and the ...
DNA binding proteins (SSBs) cover the separated strands, eliminating their attraction to each other. SSBs also protect the strands from breakage and degradation. This is an important function because single strands of DNA are less stable than double stranded DNA. Not surprisingly, they can also pr...
19.) Although the rest of the DNA-binding domain is different, the HTH motif that actually binds to the DNA is almost identical to that in the lambda CI repressor. The leucine zipper is found in many eukaryotic transcription factors, including the Fos, Jun, and Myc proteins that are ...
8.Southwestern blot hybridization of DN A-binding proteinsDNA结合蛋白的Southwestern印迹杂交 9.Histones are protein- found in eukaryotic cell nuclei, tightly bound to DNA, which has many phosphate groups.组蛋白是存在于真核细胞核中的与含很多磷酸基团的DNA紧密结合的蛋白质。 10.Prokaryotic Expression,Pur...
Transcription begins by RNA polymerase binding to a promoter sequence in DNA. In prokaryotes, accessory proteins known as specificity factors help the polymerase identify promoter sequences; in eukaryotes proteins known as transcription factors help promoter recognition and regulation of transcription. Th...
Mehta A. et.al.(1998) A sequence-specific RNA binding protein complements Apobec-1 to edit apolipoprotein B mRNA.Mol.Cel.Biol.18(8):4426-4432. Nordhoff E. et al.(1999).Rapid identification of DNA-binding proteins by mass spectrometry.Nat. ...
Telomeres are bound by dedicated proteins, which protect them from DNA damage and regulate telomere length homeostasis. In the nematode Caenorhabditis elegans, a comprehensive understanding of the proteins interacting with the telomere sequence is lacking. Here, we harnessed a quantitative proteomics approa...
DNA-Binding Proteins - chemical and physical properties, structure, melting point, boiling point, density, molecular formula, molecular weight, uses, prices, suppliers, and toxicity/safety/hazards/SDS information.