Various proteins bind in the major groove of DNA molecules and perform a specific function. Examples of DNA-binding proteins include those involved in replication, transcription, gene expression regulation, and DNA repair, among many others. Although there is great diversity in the structure and func...
DNA-binding proteins are proteins that bind to single- or double-stranded DNA, generally in the major groove if the binding is sequence-specific – as with transcription factors that regulate expression of genes, and nucleases that cleave DNA between nucleotides. DNA-binding proteins can also bind...
The structures of three proteins that regulate gene expression have been determined recently and suggest how these proteins may bind to their specific recognition sites on the DNA. One protein (Cro) is a repressor of gene expression, the second (CAP) usually stimulates gene expression, and the ...
Smad1 interacts with homeobox DNA-binding proteins in bone morphogenetic protein signaling. J Biol Chem 274: 13711-1... Bone morphogenetic proteins (BMP) transduce their signals into the cell through a family of mediator proteins known as Smads. Upon phosphorylation by the B... XM Shi,XL ...
近日来自日本名古屋大学的中野英男等人在Biotechnology and Bioengineering上发表的《Increasing cell‐free gene expression yields from linear templates in Escherichia coli and Vibrio natriegens extracts by using DNA‐binding proteins》开发了一种名为CroP-LET的方法,它通过dsDNA与单链Cro蛋白末端的结合来阻断线性...
DNA-binding proteins play an important role in most cellular processes. Therefore, it is necessary to develop an efficient predictor for identifying DNA-binding proteins only based on the sequence information of proteins. The bottleneck for constructing
Segment 2: SELEX: Identifying Target Sequences of DNA Binding Proteins SELEX:用于确定识别序列未知的蛋白结合位点 怀疑结合位点位于紫色区域,因此随机分配了ATCG核苷酸在里面,再用DNA双链与之互补。 将DNA与蛋白孵育 寻找结合了DNA的蛋白,纯化(凝胶迁移或免疫沉淀都可以做) ...
We describe a method for affinity purification of sequence-specific DNA binding proteins that is fast and effective. Complementary chemically synthesized o... JT Kadonaga,R Tjian - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 2104发表: 1986年 Spati...
The MADS domain proteins APETALA1 (AP1), APETALA3 (AP3), PISTILLATA (PI), and AGAMOUS (AG) specify the identity of Arabidopsis floral organs. AP1 and AG homocomplexes and AP3-PI heterocomplexes bind to CArG-box sequences. The DNA-binding properties of these complexes were investigated. We...
DNA-Binding Proteins structure CAS No. Chemical Name: DNA-Binding Proteins Synonyms DNA-Binding Proteins CBNumber: CB01328206 Molecular Formula: Molecular Weight: 0 MDL Number: MOL File: Mol file Request For QuotationDNA-Binding Proteins Preparation Products And Raw materials Raw ...