The starting protein of eukaryotes is the origin recognition complex (ORC), which consists of six subunits (ORC 1-6). In addition, there are cofactors Cdc6 and Cdt1. The complex has a prokaryotic initiation protein AAA+ and wing helix domain, and has a DNA binding function. Fig. 18 E....
This led us to hypothesize that the DBDs from diverse nuclear receptors can function as an NES. We tested this hypothesis by analyzing the export activity of DBDs from steroid, nonsteroid, and orphan nuclear receptors. We found that nuclear export of the GFP reporter was conferred by the DBD...
We found that the DNA-binding and FANCM-interacting functions of FAAP24, although both require the C-terminal (HhH)2 domain, can be distinguished by segregation-of-function mutations. These results demonstrate dual roles of FAAP24 in DNA damage response against crosslinking lesions, one through ...
Interestingly, distinct DNA-motifs correlated well with amino acid sequence similarity among the homeodomain proteins, revealing striking structure–function relationships. Moreover, this large-scale analysis of mouse homedomains allowed predicting DNA-binding specificities among animal homeodomains (Berger et...
Structure, function, and dynamics of the dimerization and DNA-binding domain of oncogenic transcription factor v-Myc 来自 Semantic Scholar 喜欢 0 阅读量: 174 作者:W Fieber,ML Schneider,T Matt,B Kr?Utler,R Konrat,K Bister 摘要: The protein product (c-Myc) of the protooncogene c-mycis a...
The transmembrane DNA-binding protein CadC of E. coli, a representative of the ToxR-like receptor family, combines input and effector domains for signal sensing and transcriptional activation, respectively, in a single protein, thus representing one of t
The predicted recognition motif only differs substantially at one position in the finger 4 binding site (C versus A). Supplementary Figure 14 T7EI activity profile of SpCas9MT3-ZFPTS2 at the TS2 genomic locus and OT2-2 as a function of the number of fingers. a) Both Cas9WT and Sp...
以往的研究表明，多个复制体因子具有组蛋白分子伴侣活性，并参与组蛋白到新生链的转运和分配。例如，Mcm2亚基的N末端延伸结构域（N-terminal extension，NTE）中含有一段保守的组蛋白结合结构域（histone binding domain，HBD），能够以模拟DNA结合的方式缠绕组蛋白(H3-H4)2四聚体并促进其分配至新生DNA滞后链【10,11...
The enrichment for the unique TFBS positions at the ENCODE cCREs was computed using the OLOGRAM function of thegtftkpackage (version 1.2.1) [39,72]. The same methodology was applied to the CRMs. Cell type and tissue specific enhancer expression ...
Taken together, these data illustrate the importance and multiple roles of the I domain in DNA-binding and in planta function. Results Structure of the SEPALLATA3 MI domain The MADS-box and I domain of SEP3 (SEP3MI, residues 1–90) was used in structural studies as this construct has ...