Here, we designed a hairpin DNA/RNA chimeric chain for the formation of fluorescent CuNCs and further applied it to detect reverse transcription-associated ribonuclease H (RNase H). The DNA/RNA chimera templated copper nanoclusters (DNA/RNA-CuNCs) exhibited similar spectral properties with DNA-CuN...
The extension of a primer (or positive) strand on the template (minus) strand proceeds by a rolling circle mechanism in which a “daughter” strand longer than one full length of the template circle is produced and then processed. ii. Functional concepts of polymerase: Processivity and...
possibility for spontaneous recombination between them, which would disrupt the LexA-ER-AD transcription factor and eliminate Cas9 expression. Although these events were expected to be rare, they represent about 20% of the total number of colonies that grow on inducing medium in WT cells and were ...
Two observations suggest that the 30 ends of these breaks are fairly stable and refractory to trimming; first, templated inserts were typically found very close to their cognate template, suggesting that newly made DNA was joined to the DNA that served as its template, and second, we found ...
Recent deep sequencing data confirmed the random nature of genomic integration sites [132], but the viral DNA breakpoints show a clear bias for the region around the 3 end of the minus-strand. Notably, HBV reverse transcription commonly yields a small proportion of dou...
Endogenous and exogenous factors can severely affect the integrity of genetic information by inducing DNA damage and impairing genome stability. The protection of genome integrity is ensured by the so-called “DNA damage response” (DDR), a set of evolutionary-conserved events that, triggered upon ...
The newly synthesized minus strand jumps templates from the retrotransposon RNA to the target site DNA top strand. This strand exchange is possibly facilitated by the addition of non-templated nucleotides [74] and/or the assistance of the ORF1 nucleic acid chaperone activity [16]. Plus strand ...
replication enzymes in the viral nucleocapsid is important. In addition, the targeting of the CAEV dUTPase into UNG2-deficient viral particles was able to rescue the viral infectivity, pointing out that dUTP misincorporation could be at the origin of a lethal reverse transcription process. Indeed,...
Transcription of polA mRNA is thought to be terminated within the 100 nt downstream of the polA ORF. This region contains no obvious dyad symmetries analogous to the characteristic rho-independent terminators, suggesting that the termination may be rho dependent. ...
On the other hand, our own studies provide evidence of nascent RNA-templated error-free DSBR of the transcribed genome via the transcription-coupled NHEJ (TC-NHEJ) pathway in mammalian cells (6, 21). Hence, a polymerase with reverse transcriptase (RT) activity is essential for generating the...