For reverse transcription to continue, the RNA in RNA: DNA is degraded by the RNase H activity of RT, allowing the minus strand strong-stop DNA to undergo the “first jump” by hybridizing to the 3′-end of the RNA genome. RT continues extension of the minus strand DNA while it also ...
Collisions of the transcription and replication machineries on the same DNA strand can pose a significant threat to genomic stability. These collisions occur in part due to the formation of RNA-DNA hybrids termed R-loops, in which a newly transcribed RNA
1 A). RT initiates minus strand DNA synthesis by elongation of a specific cellular tRNA primer annealed to the primer binding site (PBS) and continues as far as the 5′ end of the RNA genome, generating the so-called strong stop cDNA (ss-cDNA). The concomitant degradation of the RNA ...
An on-column treatment with DNaseI was also performed. After washing, RNA was eluted in 40 μl RNase-free H2O. RNA was quantitated using the Qubit RNA HS Assay Kit. We performed first strand synthesis on each replicate with Maxima H Minus Reverse Transcriptase. We mixed 2 μg of total ...
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positive regulation of transcription by RNA polymerase II, vegetative to reproductive phase transition of meristem, regionalization, response to auxin, negative regulation of transcription (DNA-templated), and response to light stimulus), while three GO terms were underrepresented (cellular metabolic process...