Kadonaga 教授发表在Nature Structural & Molecular Biology 的观点文章The transformation of the DNA template in RNA polymerase II transcription: a historical perspective 进行了编译,以飨读者。 RNA聚合酶I、II和III的发现开辟了基因表达的新时代。在这篇文章中,James结合自己实验室的发展,回忆了DNA模板在RNA...
During transcription, the template strand, also known as the ‘minus’ or ‘antisense’ strand, is generated byRNApolymerase itself in the case ofprokaryotes, or by a transcription factor with helicase activity in case of eukaryotes. The RNA polymerase binds a promoter sequence in DNA to initiate...
Transcription facilitated genome-wide recruitment of topoisomerase I and DNA gyrase. PLoS Genet. 13, e1006754 (2017). Article PubMed PubMed Central CAS Google Scholar Liu, L. F. & Wang, J. C. Supercoiling of the DNA template during transcription. Proc. Natl. Acad. Sci. USA. 84, ...
【2】Transcription is the first stage of the expression of genes into proteins. In transcription,an mRNA (messenger RNA) intermediateis transcribed from one of the strands of the DNA molecule. 转录是基因表达为蛋白质的第一阶段。在转录...
GapR is a nucleoid-associated protein required for the cell cycle of Caulobacter cresentus. We have determined new crystal structures of GapR to high resolution. As in a recently published structure, a GapR monomer folds into one long N-terminal α helix
. The PCR-amplified linear DNA can be used directly in the coupled transcription–translation, with the cell-free system with low nuclease activity. This is one of the advantages of the cell-free method over the recombinant expression methods. The optimal DNA template concentration for coupled ...
Enzymatic ligation methods are useful in diagnostic detection of DNA sequences. Here we describe the investigation of nonenzymatic phosphorothioate-iodide ... Xu,Yanzheng,Karalkar,... - 《Nature Biotechnology》 被引量: 84发表: 2001年 Template-independent ligation of single-stranded DNA by T4 DNA ...
18 A DNA-Templated Library of Synthetic Molecules DNA-templated Reagent library (1) synthesis Sequence First Round specific Starting template annealing Coded first round library products Reagent library (3) Reagent library (2) Sequence specific Sequence specific annealing and synthesis Coded second round...
1月2日,清华大学生命学院孙前文副教授实验室在《自然·通讯》(Nature Communications ) 期刊上发表了题为“引物酶促进转录和复制在同一模板链上的竞争从而导致DNA损伤”(Primase promotes the competition between transcription and replication on the same template strand resulting in DNA damage)的研究论文。该研究揭...
网络核酸为模板 网络释义 1. 核酸为模板 用PCR检测的原理是藉由具有专一性的引子(specific primer),以待测检体核酸为模板(DNA template), 经由DNA聚合?(… blog.udn.com|基于4个网页 例句 释义: 全部,核酸为模板