The data show RNA purified from TRIzol® samples using the Direct-zol™ RNA MiniPrep compared to a method known to be unbiased2 (mirVana™, Ambion). MicroRNA analysis was performed using miRNA- seq (MiSeq®, Illumina) and direct hybridization assay (nCounter®, Nanostring). 1 ...
Direct-zol™ RNA MiniPrep w/ TRI-Reagent®货号:A-R2051 背景资料:The Direct-zol™ RNA MiniPrep provides a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI-Reagent® or similar*. Total RNA (including small and non...
R2060,R2061,R2063 Direct-zol RNA MicroPrep 下一个 D4068&D4069&D4074&D4075&D4081 Quick-DNA™ Miniprep Plus Kit 产品介绍 产品说明书 特点: 1. 样品来源:TRIZOL等类似试剂裂解的样品。 2. 样品范围:≥17核苷酸。 3. RNA纯度:高质量的RNA可应用于高通量测序等敏感的下游实验。
Direct-zol RNA MiniPrep 试剂盒是 RNA 纯化试剂盒,可提供一种简化的方法,可直接从 TRI Reagent 或类似试剂中的样品中纯化高达 50 µg(每次制备)的高质量 RNA。总RNA,包括小RNA(17-200 nt),可从多种样品来源(细胞、组织、血清、血浆、血液、生物液体等)中有效分离。通过常规相分离 RNA 被证明可以选择性富...
Q6: Is it possible to extract proteins with the Direct-zol RNA kits? Yes, proteins can be Acetone Precipitated post RNA binding step. Please request supplementary protocol from Zymo Research Technical Support. Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?
Total RNA was purified from homogenized samples with the Direct-Zol RNA miniprep protocol (Zymo), DNase treated with TURBO DNase (Life Technologies), and 3′ dephosphorylated with T4 Polynucleotide Kinase (New England Biolabs). rRNA was removed with the bacterial Ribo-Zero rRNA removal kit (...
Cells were lysed in Tri-reagent (Zymo) and total RNA was extracted using Direct-zol RNA Miniprep kit (Zymo) according to the manufacturer’s instructions. RNA was quantified, and RNA integrity was checked by Tapestation 4150 (Agilent). Only samples with an RNA integrity number above 8.0 were...
Protocol: Datasheet: SDS (MSDS): SDS (MSDS): R2070T | TRI Reagent Supplemental Info. Tips & Tricks For RNA Isolation How to Extract RNA from TRIzol® How to Design Primers for PCR Experiments What is RNA-Seq? FAQ Q1: Is Direct-zol suitable for very small numbers of cells?
RNA isolation was performed using the Direct-zol RNA Miniprep kit (Zymo Research). RNA was prepared for small RNA-seq according to the following protocol. In brief, no more than 5 µg total RNA was treated with DNase I (NEB) for 30 min at 37 °C then purified using the RNA...
Direct-zol™ RNA MiniPrep (ZYMO RESEARCH) was used and performed by according to manufacturer's instructions. The entire concentration and A260/A280 ratio of mRNA were measured with an ND-1000 (NanoDrop). Reverse transcription PCR One-step reverse transcription PCR was performed with a RevertAid...