The data show RNA purified from TRIzol® samples using the Direct-zol™ RNA MiniPrep compared to a method known to be unbiased2 (mirVana™, Ambion). MicroRNA analysis was performed using miRNA- seq (MiSeq®, Illumina) and direct hybridization assay (nCounter®, Nanostring). 1 ...
The Direct-zol™ RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated....
Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus? Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit. Q13: I ran out of RNA Wash Buffer. Can I use something else?
Total RNA extraction from 5 different samples of ∼100 gastrulating yw embryos each injected or not was performed using the Direct-zol RNA miniprep kit (Zymo Research, R2050) plus a on-column DNAse I treatment to remove genomic DNA contamination as recommended. Reverse transcription was ...
RNA isolation was performed using the Direct-zol RNA Miniprep kit (Zymo Research). RNA was prepared for small RNA-seq according to the following protocol. In brief, no more than 5 µg total RNA was treated with DNase I (NEB) for 30 min at 37 °C then purified using the RNA...
Samples were centrifuged for 5 min at 12,000 × g, and RNA was isolated from the supernatant using a Direct-zol RNA MiniPrep kit (Zymo Research, Irvine, CA). RNA integrity and quantity were measured using a 6000 Nano LabChip kit on a Bioanalyzer 2100 (Agilent, Santa Clara, CA) and a...
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德国QIAGEN,货号:27361,DirectPrep 96 Miniprep Kit (4) 价格电议 起批量≥1 盒 最小起订1盒 供货总量66盒 发货地址广东省深圳市 建议售价￥0/盒 更新日期2025年01月15日 即时洽谈立即询价查看联系方式 收藏产品发送留言 VIP9年 深圳子科生物科技有限公司 ...
It has been shown that TET2 expression is often silenced post-transcriptionally in human cancers, such as by mircroRNA-mediated gene silencing. Previous studies have revealed that TET2 can be a direct target of the Let-7adf cluster in LPS-activated macrophages33, it can be suppressed by miR-...
The Tecan MiniPrep 60 MolBio Work- station was used with the Eppendorf Perfectprep Plasmid 96 Vac Direct Bind kit to isolate 96 plasmid DNA samples from high and low copy plasmids. Six different plasmids, each in a different bacterial host strain, were processed using a proto- col written ...