The Direct-zol™ RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated....
Direct-zol (log10) Direct-zol (log10) MicroRNA isolation using Direct-zol™ RNA kits. The data show RNA purified from TRIzol® samples using the Direct-zol™ RNA MiniPrep compared to a method known to be unbiased2 (mirVana™, Ambion). MicroRNA analysis was performed using miRNA- ...
R2060,R2061,R2063 Direct-zol RNA MicroPrep 下一个 D4068&D4069&D4074&D4075&D4081 Quick-DNA™ Miniprep Plus Kit 产品介绍 产品说明书 特点: 1. 样品来源:TRIZOL等类似试剂裂解的样品。 2. 样品范围:≥17核苷酸。 3. RNA纯度:高质量的RNA可应用于高通量测序等敏感的下游实验。
Zymoclean? Gel RNA Recovery Kit ZR small-RNA? PAGE Recovery Kit OneStep? PCR Inhibitor Removal Kit OneStep?-96 PCR Inhibitor Removal Kit Direct-zol? RNA MiniPrep Direct-zol RNA MiniPrep w/ TRI Reagent Direct-zol?-96 RNA Direct-zol?-96 RNA w/ TRI Reagent Direct-zol?-96 MagBead RNA ?
epithelial cells using the Direct-zol™ RNA MiniPrep compared to a DNA containing preparation from Supplier Q (1% agarose/TAE). (B) Small RNAs are effectively recovered with the Direct-zol™ procedure while absent in Supplier Q preparations (Agilent Bioanalyzer 2100, Small RNA Chip data shown...
Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus? Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit. Q13: I ran out of RNA Wash Buffer. Can I use something else?
RNA isolation was performed using the Direct-zol RNA Miniprep kit (Zymo Research). RNA was prepared for small RNA-seq according to the following protocol. In brief, no more than 5 µg total RNA was treated with DNase I (NEB) for 30 min at 37 °C then purified using the RNA...
Total RNA was extracted from cells in exponential phase growing at 37°C in LB medium using a Direct-zol RNA MiniPrep kit with TRI-Reagent (Zymo Research). DNase treatment was carried out using a Turbo DNA-free kit (Ambion, Inc.). Ribosomal RNA was depleted using a Ribo-Zero rRNA ...
RNA sequencing Total RNA was extracted from HeLa cells using the Direct-zol RNA Miniprep Kit (Zymo, R2052). The integrity of the RNA (RNA integrity number; RIN) was evaluated using the Agilent 2100 Bioanalyzer. Subsequently, indexed cDNA libraries were generated with the TruSeq stranded mRNA ...
It has been shown that TET2 expression is often silenced post-transcriptionally in human cancers, such as by mircroRNA-mediated gene silencing. Previous studies have revealed that TET2 can be a direct target of the Let-7adf cluster in LPS-activated macrophages33, it can be suppressed by miR-...