At different times of culture, IL-1β production was quantified by ELISA in cell supernatants and expression of pro- and anti-inflammatory genes (IL-1β, IL-6, TNF-α, IL-8, COX-2, IL-10) was determined by quantitative PCR (qPCR). In vivo CPP crystal-mediated inflammation was ...
www.nature.com/scientificreports OPEN Pre-exposure of Arabidopsis to the abiotic or biotic environmental stimuli "chilling" or "insect eggs" received: 11 January 2016 exhibits different transcriptomicaccepted:03June2016 responses to herbivoryPublished:22June2016 Vivien Firtzlaff1,*, Jana ...
Figure 3 Comparison of mRNA expression of related markers in PDLSCs among groups 图3 qRT-PCR分析成骨分化相关基因的表达*为实验组与Control组相比,P < 0.05,*数量不同,代表实验组的组间比较差异有统计学意义,P < 0.05。Satb2:AT序列结合蛋白2,Runx2:核心结合因子2,OCN:骨钙素 Full size|PPT slide 2....
RNA Isolation and Quantitative RT-PCR. Total RNA and miRNA were extracted from TKE2 and MCE cells using TRIzol reagents (Invitrogen, Carlsbad, CA). Reverse transcription of the extracted RNA into cDNA were conducted using the Transcriptor First-Strand cDNA synthesis kit (TaKaRa, Shiga, Japan...
Molecular cloning and sequence analysis of Lv-ILF2 Five full-length cDNAs for Lv-ILF2 transcripts were obtained from the shrimp muscle by using the RACE approaches. The composite sequences were further confirmed by PCR amplification, cloning and sequencing. As shown in Fig. 1, the five isoforms...
of aromatic volatiles by constructing regulatory networks; the key candidate genes were validated using quantitative real-time PCR (qRT-PCR). These results will be helpful in identifying the characteristic aroma ofC. cassiabarks and providing insights into the regularity of aromatic VOC formation inC....
PCR amplification of the 16S rRNA gene at the V3-V4 region was performed using the universal prokaryotic primers 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3′) with appended adaptor and barcodes sequences as previously reported26,27. Briefly, each PCR react...
A syndrome has recently been described4,5 of progressive outer retinal necrosis (PORN) in patients with AIDS. In the first report herpes virus particles were identified on retinal biopsy in one of two cases and by the polymerase chain reaction (PCR) in the other. In the second report, VZV...
energies Article Thermal Response Testing Results of Different Types of Borehole Heat Exchangers: An Analysis and Comparison of Interpretation Methods Angelo Zarrella 1,*, Giuseppe Emmi 1, Samantha Graci 1, Michele De Carli 1, Matteo Cultrera 2, Giorgia Dalla Santa 2, Antonio Galgaro 2, David ...
[68] under the following conditions: 30 s at 98 °C; 35 cycles of denaturation at 98 °C for 10 s, annealing at 54 °C for 30 s, extension at 72 °C for 45 s; a final step at 72 °C for 10 min. The PCR reactions were conducted in a 25 μL mixture containing 12.5 μL ...