PCR is a method used to amplify DNA from a small amount of DNA template. RT-PCR uses reverse transcription to produce a DNA template from an RNA source that can then be amplified. PCR and RT-PCR are typically endpoint reactions, while qPCR and RT-qPCR use the kinetics of the rate of ...
PCR (polymerase chain reaction) is a widely used molecular biology technique that’s used for the amplification and detection of DNA and RNA sequences. RT-PCR, qPCR, and R-qPCR are all variations of the basic PCR technique. PCR The traditional PCR technique is the simplest...
Thus, aside from sex differences in immune changes during myocarditis, which have been well characterized in the past, the main difference in cardiac transcript enrichment between males and females with myocarditis occurred in pathways related to mitochondrial function. Fig. 4 Sex differences in ...
1, HUVECs were transfected 48 h before calcein-AM loading and overnight serum deprivation. The knockdown of PTX3 expression was analyzed by reverse transcription and quantitative real-time PCR (RT-qPCR) and by ELISA measurement of the secreted PTX3 protein. Total RNA extraction and RT-qPCR ...
Errors of omission and premature responses that occurred during the intertrial interval were also calculated and the number of all total errors (perseverative, regressive, random, omission, and premature responses) were compared between groups. In addition, the duration to complete the strategy ...
We found significant (p = 0.001, PERMANOVA) community composition differences (Supplementary TableS1) in the intestinal microbiome between the two cohorts when using unweighted UniFrac (Fig.1c, left) distance as well as Bray–Curtis (p = 0.001, PERMANOVA) and weighted UniFrac (p =...
We used the EvoScript RNA SYBR® Green I Master (Roche, Germany), a one-step RT-PCR kit that enabled both reverse transcription of RNA into cDNA and qPCR to be done in a single reaction mixture. The reactions were performed using the QuantStudio™ 6 Flex Real-Time PCR System. ...
The concentration of each cDNA library was estimated with qPCR (KAPA-PCR) using Illumina sequencing primers (KAPA Biosystems catalog # KK4835), and average frag- ment length was determined with a bioanalzyer high sen- sitivity DNA chip (Agilent Technologies, catalog #5076– 4626). 12nM of ...
Here, we investigated the functional differences between ANT and both AIL5 and AIL7 by expressing these two AILs under the control of the ANT promoter. We found that only ANT:gAIL5 lines with much higher amounts of AIL5 mRNA as compared with ANT could compensate for loss of ANT function...
In this systematic review, we highlight the differences between the male and female zebrafish brains to understand their differentiation and their use in studying sex-specific neurological diseases. Male and female brains display subtle differences at th