Molecular difference between WASP and N-WASP critical for chemotaxis of T-cells towards SDF-1α Neeraj Jain & Thirumaran Thanabalu Wiskott-Aldrich Syndrome protein (WASP) integrates cell signaling pathways to the actin cytoskeleton, which play a critical role in T-cell activation and ...
RT-qPCRMicroRNA-21 (miR-21) overexpression is characteristic for various types of tumors, but it is still unknown whether its expression levels differ between invasive and non-invasive breast carcinomas. The main goal of the study was to determine the difference in miR-21 expression among ...
Transcripts were quantified using a combination of competitive reverse transcriptase PCR (RT-PCR) and t-RFLP. In addition to pmoA, we used mmoX encoding for a subunit of the sMMO to search for genes and transcripts of aerobic MOB that lack a pmoA. Three different primer sets were applied ...
Figure 02: cGMP in signal transduction pathway What is the difference between cAMP and cGMP? cAMP vs cGMP Summary – cAMP vs cGMP cAMP and cGMP are hydrophilic cyclic nucleotides important in cells as second messengers in cell communication. These molecules receive and pass signals from receptors ...
2.7. Reverse Transcription-Quantitative PCR (RT-qPCR) The mRNA levels of selected genes were analyzed by RT-qPCR. For mRNA expression in mouse RPE, total RNA was prepared by the two-step extraction method as described above. First-strand cDNA was synthesized from 200 ng of total RNA with ra...
The first-strand cDNA was synthesized with 1 μg RNA by using FastKing RT Kit (With gDNase) (Tiangen, Beijing, China). The Talent qPCR PreMix (SYBR Green) (Tiangen, Beijing, China) was used for qRT-PCR by a Bio-Rad CFX Connect Real-Time System machine (Bio-Rad, Hercules, CA, USA...
2.10. qPCR Analysis Total RNA was reverse transcribed to generate the first strand cDNA using a PrimeScript™ RT reagent Kit with gDNA Eraser (Takara, Kyoto, Japan, RR047B). The cDNA was diluted 1:4 in distilled water and used as template for qPCR. The Maca actin gene (LmActin) was ...