Discover an array of solutions to optimize, execute, and troubleshoot MIQE-compliant real-time PCR experiments.
However, when conducting PCR, there are a number of additional considerations that are frequently overlooked when investigating the presence or amount of nucleic acid within a sample.Jim HuggettUniversity College London
Thermo Fisher offers learning resources on Real-Time PCR basics. Learn the advantages of multiplexing, duplexing and how to perform multiplex qPCR to measure the expression of three or four genes simultaneously. Read about considerations when multiplexin
The percentage of 2CLCs was quantified by FACS. The mean ± s.d. of the indicated number of replicates is shown. P values were calculated by two-sided Mann–Whitney test. d, Quantitative polymerase chain reaction (qPCR) analysis upon transfection of siRNA for Crabp1 and RA treatment. ...
PCR试剂盒设置 qPCR 反应(20 μL 体系,在样品制备室进行) 9.在 PCR 管中加入下列成分(待测样品需要做三次重复,下表只列出一次。样品管和阴性对照设置完毕后才设置阳性对照,并且阳性对照样品要等所有管子盖上盖子储存好后*后加): 注:仅 ABI7500、7700 和 7900 仪器 需要使用 ROX 作为对照,其他荧光 PCR 仪器...
We selected six NFIB-bound loci from this set of 523 peaks and validated this binding by ChIP-qPCR (Supplementary Fig. 1g). To test whether methylation of NFIB affects transcription regulation, we compared the mRNA levels of these selected genes upon the overexpression of wild-type NFIB (NFIB...
4. 一管式荧光定量PCR检测,避免后续污染。 5. 本试剂盒足够50次20μL反应体系的荧光定量PCR。 本产品只适用于科研,不能用于临床诊断。 相关产品: 马铃薯内标准UDP-葡萄糖焦磷酸酶基因LAMP试剂盒 马铃薯内标准UDP-葡萄糖焦磷酸酶基因PCR试剂盒 马铃薯内标准UDP-葡萄糖焦磷酸酶基因染料法qPCR试剂盒 马铃薯内标准UDP...
Total RNAs were extracted using TRIZOL reagent (Invitrogen). Real-time qPCR was performed using the StepOnePlus Real-time PCR system (Applied Biosystems) with SYBR Green PCR Master Mix (Transgen, Beijing, China) as previously described16. The primers were designed using Primer-BLAST (National Cen...
Quantitative real-time PCR to assess viral titer and antimicrobial products Whole lungs and 1 cm of proximal duodenum, terminal ileum and proximal colon were collected 5 to 7 days after infection. Intestinal pieces were wash with PBS and all organs were transferred in Eppendorf tubes containing ...
Real-time PCR (qPCR) is a very common technique for detecting and quantifying various bacterial species from different sources, like food (De Martinis et al., 2007), rumen (Tajima et al., 2001) or faeces (Rinttila et al., 2004). This method is highly sensitive and allows for ...