(A) WASP-I30 region was inserted in N-WASP between WH1 domain and basic region of N-WASP. (B) Jurkat T-cells expressing EGFP tagged WASPR, WASPRΔI30, N-WASP and N-WASP-I30 were stimulated on anti-CD3 (OKT3)-coated coverslip for 5 min and stained with Alexa fluor 594 ...
In this work, we first visualized the PGC migration route by injecting the mRNA containing GFP CDS and 3′UTR of vasa, and counted the number of PGCs in XX female and XY male embryos of yellow catfish. And the result showed that the XX female embryos had more PGCs than XY male ...
preventing the miRNA from cleaving without altering its amino acid sequence, and cloned it into pFGC-eGFP via overlapping PCR. The method of coexpression of Bra-miR319a andBcMYB101was described previously [6]. After 48 h of transfection, GFP fluorescence was detected using a confocal laser sca...