Schematic diagram of cloning the gene of interest containing internal restriction sites into expression vector.Jiancai WangRonghua XuAizhong Liu
The full library of constructs for recombinant expression was assembled by a combination of cloning and gene synthesis. For cloned sequences, cDNA templates (OriGene) were amplified by PCR with primers delineating the extracellular domain. Overhangs on the primers introduced NotI and AscI restriction...
A single cytosine deletion in the OsPLS1 gene encoding vacuolar-type H+-ATPase subunit A1 leads to premature leaf senescence and seed dormancy in rice TheOsPLS1locus that encodes VHA-A1 in rice, was identified by map-based cloning.OsPLS1/VHA-A1is involved in premature leaf senescence and se...
- 《Gene An International Journal Focusing on Gene Cloning & Gene Structure & Function》 被引量: 0发表: 2023年 Erratum to: Biodegradation of Dichlorodiphenyltrichloroethanes (DDTs) and Hexachlorocyclohexanes (HCHsc) with Plant and Nutrients and Their Effects on the ... Erratum to: Microb Ecol...
Genome mapping and gene analysis of Antheraea pernyi nucleopolyhedrovirus for improvement of baculovirus expression vector system We have constructed a genome DNA map of the I fragments of viral DNA were obtained by shotgun cloning, and both termini of each cloned fragment were sequen... YJ Huang,...