The transformation efficiencies of DH5α competent cells and 5-alpha competent E. coli (NEB) were compared using pUC19 DNA and following the manufacturers’ recommended protocols. The transformation efficiency specification for DH5α competent cells is 1.0 x 109 ...
Blue/white screening withlacZ∆M15 High insert stability due torecA1 mutation High yield and quality of DNA due toendA mutation High transformation efficiency (Figure 1) Click image to enlarge Figure 1. Comparing transformation efficiencies of DH5α competentE. coliwith ...
MAX Efficiency DH5αF´IQ Competent Cells have the lacZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal. They also carry the recA1 mutation which helps to reduce the rate of recombination while propagating plasmid DNA and the endA1 mutation that ...
Figure 1. Comparing transformation efficiencies of DH5α competent E. coli with NEB 5-alpha 5α and Zymo 5a in similar product configurations. Transformation efficiency was measured by performing triplicate transformations, using 10 pg pUC19 DNA, according to manufacturer’s...
MAX Efficiency DH5αF´IQ Competent Cells have the lacZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal. They also carry the recA1 mutation which helps to reduce the rate of recombination while propagating plasmid DNA and the endA1 mutation that ...
Figure 1. Comparing transformation efficiencies of DH5α competent E. coli with NEB 5-alpha 5α and Zymo 5a in similar product configurations. Transformation efficiency was measured by performing triplicate transformations, using 10 pg pUC19 DNA, according to manufacturer’s...
MAX Efficiency DH5αF´IQ Competent Cells have the lacZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal. They also carry the recA1 mutation which helps to reduce the rate of recombination while propagating plasmid DNA and the endA1 mutation that ...
Blue/white screening withlacZ∆M15 High insert stability due torecA1 mutation High yield and quality of DNA due toendA mutation High transformation efficiency (Figure 1) Click image to enlarge Figure 1. Comparing transformation efficiencies of DH5α competentE. coliwith...
Product Information Sheet: MAX Efficiency DH5alpha Competent Cells 常見問答集 (常見問題) Components of the ligation reaction (enzymes, salts) can interfere with transformation, and may reduce the number of recombinant colonies or plaques. We recommend a five-fold dilution of the ligation mix, and...