NEB® 5-alpha E. coli 感受态细胞(高效级) NEB 5-alpha E. coli 感受态细胞是广泛使用的 DH5α 的衍生菌株。它具有 T1 噬菌体抗性且属于 endA 缺陷型,可用于高质量质粒的制备。 高转染效率菌株,是多种应用的理想选择 有多种规格可供选购,包括一次性管、200 μL 管、96 和 384 孔板和 12 x 8 联...
图1.DH5α 感受态细胞与 NEB 5-alpha 和 Zymo 5a 感受态细胞的转化效率比较。按照生产商推荐的方案,使用 10 pg pUC19 DNA 进行三次转化,测定转化效率。每次转化均进行两个重复。 有关基因型的信息,请查看E. coli感受态细胞的基因型和遗传标记 E. coliDH5α 感受态细胞菌株 ...
Testing was performed on many of our chemically competent cells in both the MultiShot StripWell and MultiShot FlexPlate format with a shortened transformation protocol. Use of a rapid transformation protocol (add DNA, no heat shock, no recovery period, plate on warm agar) yielded up to a log...
This paper reports the effect of shaking during or after the heat shock of competent DH5-Alpha strain of E. coli, induced by CaCl2. Our results show that shaking during heat shock does not have any positive impact on the transformation efficiency. On the other hand, after the heat shock,...
coli DH5α can be achieved through α-complementation mechanism by introducing a plasmid carrying a LacZ alpha subunit into the E. coli DH5α strain, which therefore complements the truncated LacZ gene and produces an active β-galactosidase enzyme [23]. Here, we study the α-complementation ...
翻译结果3复制译文编辑译文朗读译文返回顶部 Application PCR technology, with special specific introduction real spread increased out sheep pox virus structure protein BKLP2 gene, connection Yu pMD-l8-T carrier, transformation to feel State cell DH5 Alpha in the, on restructuring mass grain double enzym...
The English word has many uses, as an adverb and conjunction, but its principal sense underwent an almost subtle transformation during the twentieth century. Yet used to mean what still still means: roughly, ``now as until now.'' That is its sense in Francis Scott Key's words ``Oh say...
NEB® 5-alpha E. coli 感受态细胞(高效级) NEB 5-alpha E. coli 感受态细胞是广泛使用的 DH5α 的衍生菌株。它具有 T1 噬菌体抗性且属于 endA 缺陷型,可用于高质量质粒的制备。 高转染效率菌株,是多种应用的理想选择 有多种规格可供选购,包括一次性管、200 μL 管、96 和 384 孔板和 12 x 8 联...
Figure 1. Comparing transformation efficiencies of DH5α competentE. coliwith NEB 5-alpha 5α and Zymo 5a in similar product configurations.Transformation efficiency was measured by performing triplicate transformations, using 10 pg pUC19 DNA, according to manufacturer’s rec...
Figure 1. Comparing transformation efficiencies of DH5α competent E. coli with NEB 5-alpha 5α and Zymo 5a in similar product configurations. Transformation efficiency was measured by performing triplicate transformations, using 10 pg pUC19 DNA, according to manufacturer’s...