NEB® 5-alpha E. coli 感受态细胞(高效级) NEB 5-alpha E. coli 感受态细胞是广泛使用的 DH5α 的衍生菌株。它具有 T1 噬菌体抗性且属于 endA 缺陷型,可用于高质量质粒的制备。 高转染效率菌株,是多种应用的理想选择 有多种规格可供选购,包括一次性管、200 μL 管、96 和 384 孔板和 12 x 8 联...
This paper reports the effect of shaking during or after the heat shock of competent DH5-Alpha strain of E. coli, induced by CaCl2. Our results show that shaking during heat shock does not have any positive impact on the transformation efficiency. On the other hand, after the heat shock,...
Please see the product manual for full details on this rapid transformation protocol option. The MultiShot FlexPlate is a PCR plate and can be placed in a thermal cycler or heat block set at 42 degrees C to transform the cells by the heat shock method. The duration of the heat shock ...
Figure 1. Comparing transformation efficiencies of DH5α competent E. coli with NEB 5-alpha 5α and Zymo 5a in similar product configurations. Transformation efficiency was measured by performing triplicate transformations, using 10 pg pUC19 DNA, according to manufacturer’s...
coli DH5α can be achieved through α-complementation mechanism by introducing a plasmid carrying a LacZ alpha subunit into the E. coli DH5α strain, which therefore complements the truncated LacZ gene and produces an active β-galactosidase enzyme [23]. Here, we study the α-complementation ...
翻译结果3复制译文编辑译文朗读译文返回顶部 Application PCR technology, with special specific introduction real spread increased out sheep pox virus structure protein BKLP2 gene, connection Yu pMD-l8-T carrier, transformation to feel State cell DH5 Alpha in the, on restructuring mass grain double enzym...
The English word has many uses, as an adverb and conjunction, but its principal sense underwent an almost subtle transformation during the twentieth century. Yet used to mean what still still means: roughly, ``now as until now.'' That is its sense in Francis Scott Key's words ``Oh say...
To achieve the highest possible transformation efficiency with Thermo Scientific DH5α (Cat. No. EC0112) and DH10B (Cat. No. EC0113) competent cells, please follow the transformation protocol provided with the product and pay attention to the following details: - Make sure to use wet well...
Figure 1. Comparing transformation efficiencies of DH5α competentE. coliwith NEB 5-alpha 5α and Zymo 5a in similar product configurations.Transformation efficiency was measured by performing triplicate transformations, using 10 pg pUC19 DNA, according to manufacturer’s reco...
Figure 1. Comparing transformation efficiencies of DH5α competent E. coli with NEB 5-alpha 5α and Zymo 5a in similar product configurations. Transformation efficiency was measured by performing triplicate transformations, using 10 pg pUC19 DNA, according to manufacturer’s...