We propose that the DNA binding differences between MCM2-7 and MCM467 correspond to a conformational change at the MCM2/5 active site with putative regulatory significance. 展开 关键词: Saccharomyces cerevisiae DNA Helicases Adenosine Triphosphate DNA, Single-Stranded Nucleotides DNA-Binding Proteins ...
mRNA translation in axons eNAbles neurons to introduce new proteins at sites distant from their cell body. mRNA-protein interactions drive this post-transcriptioNAl regulation, yet knowledge of RNA binding proteins (RBP) in axons is limited. Here we used proteomics to identify RBPs interacting with...
Laraki G et al (2008) Interactions between the double-stranded RNA- binding proteins TRBP and PACT define the Medipal domain that mediates protein-protein interactions. RNA Biol 5(2):92-103Laraki G,Clerzius G,Daher A,et al.Interactions between the double-stranded RNA-binding proteins TRBP ...
AR1 and BR1 have similar nucleic acid binding affinities, suggesting a possible mechanism for the observed effects of the viral coat protein on viral movement. 展开 关键词: Geminiviridae Capsid Plant Leaves Vegetables Viral Proteins DNA, Single-Stranded Base Sequence Phenotype Mutation Suppression, ...
4d,e). PARP1 Y889 is within the drug-binding pocket in the catalytic domain (Fig. 4d) and has been shown to be a critical residue for determining inhibitor specificity over related proteins such as tankryase 1 through van der Waals and pi-interactions with PARP inhibitors60. This ...
Sliding clamp proteins are used by DNA polymerases as mobile tethers for highly processive replication of DNA. By themselves, the polymerases can incorporate a few nucleotides into the growing DNA polymer in a single binding event; in association with clamps, however, their processivity can increase...
Checkpoint immunotherapy (CPI) has increased survival for some patients with advanced-stage bladder cancer (BCa). However, most patients do not respond. Here, we characterized the tumor and immune microenvironment in pre- and post-treatment tumors from t
the 42 nucleotide strand of IRL, with a single nucleotide (C) at its 5' end representing the spacer base between the two abutted ends. The result of the annealing reaction was a double-stranded MCJ with a one base pair spacer, nicked at the CA-3' terminus of the IRR CD. Very effici...
Inhibition of Transcriptional Activation of Brn3-Proteins Double-stranded oligonucleotides were prepared from complementary single stranded phosphorothioate-bonded fluorescence-labeled oligonucleotides by melting at 95° C. for 5 min followed by a cooling phase at room temperature. The sequences of the ol...
NS2B–3181- and NS3181–green fluorescent protein fusion proteins were used to determine the intracellular distribution of the protease complex. The former localized in membrane-based vesicular structures, whereas the latter localized poorly. The data suggest that NS2B–NS3 complex formation requires ...