CALABRO, VALERIEHAYDEN-LEDBETTER, MARTHA SUSANTCHISTIAKOVA, LIOUDMILATHOMPSON, PETER ARMSTRONGSCHULER, ALWINBADER, LIOUDMILAGROSMAIRE, LAURA SUEBRADY, WILLIAMFOLLETTIE, MAXIMILIAN T.LEDBETTER, JEFFREY A.
Single-Stranded DNA-Binding Proteins The single-stranded DNA of the lagging strand created by the action of the helicase is covered by tightly binding single-stranded DNA-binding proteins. In E. coli, this protein is a tetramer known as single-stranded-binding (SSB) protein, which the ssDNA ...
Figure 7. Single-strand DNA-binding proteins of bacteria and eukaryotes. (a) Model of E. coli SSB tetramer-ssDNA complexes based on crystal structure information. SSB is a homotetramer of subunits (colored differently); each subunit has one OB fold. The SSB tetramer has two binding modes, ...
As a result, A3A-induced mutations are enriched at hairpin-forming regions of cancer genomes and show strand bias for replication intermediates. It is also possible that larger ssDNA binding proteins, like RNA Polymerase occlude A3A binding. In our in vitro transcription assay, a 99 kDa T7 ...
Single-strand DNA (ssDNA)-binding proteins (SSBs) are ubiquitous and essential for a wide variety of DNA metabolic processes, including DNA replication, recombination, DNA damage detection and repair. SSBs have multiple roles in binding and sequestering ssDNA, detecting DNA damage, stimulating nuclease...
Single-stranded DNA-binding proteins (SSBs) play essential roles in DNA replication,recombinational repair,and maintenance of genome stability.In human,the major SSB,replication protein A (RPA),is a stable heterotrimer composed of subunits of RPA1,RPA2,and RPA3,each df which is conserved not ...
3National Center for Protein Science Shang- hai, 320 Yueyang Road, Shanghai 200031, China Human single-strand (ss) DNA binding proteins 1 and 2 (hSSB1 and 2) are components of the hSSB1/2-INTS3- C9orf80 heterotrimeric protein complex shown to participate in DNA damage response and maint...
更多“Single-stranded binding proteins (SSB) bind to the outer backbone of DNA during replication. What wo…”相关的问题 第1题 单链结合蛋白(SSB,single-strand binding protein) 点击查看答案 第2题 An old Indian story says that the game of chess was invented by Sissa Ben, prime Minister ...
Exonucleases are enzymes involved in DNA repair mechanisms that hydrolyze one strand of DNA producing single-stranded DNA and free nucleotides. Here we present a study on the effect of single-stranded DNA binding proteins on the reaction of lambda-exonuclease with lambda-DNA that has been fluoresce...
To elucidate the mechanism of DNA binding, we generated ZNF827 mutant proteins in which either ZnF cluster was deleted (ZnFΔ1-3, ZnFΔ4-9), and a severely truncated ZNF827 mutant protein, which includes the removal of both ZnF domains (Truncated) (Fig.1Aand Supplementary Fig.1E). EMSAs...