a, Summary of the strain-specific gene sets showing the number of genes broken down by GENCODE biotype.b, Heterozygous SNP (hSNP) density for a 50 Mb interval on chromosome 11 in 200 kb windows for 17 inbred mouse strains based on sequencing read alignments to the C57BL/6J (GRCm38) re...
However, the methods we have been using for most of the last 10 years will not yield even one complete open reading frame (ORF) for every gene—the first plateau on the long climb toward a comprehensive catalog. These strategies—sequencing randomly selected cDNA clones, aligning protein ...
To study associations between genetic variants and morphological traits, we assembled a cohort of iPSC lines from 297 unique donors for which we generated image-based profiling and whole-genome sequencing data (Fig.1). We obtained pre-derived cell lines from the CIRM iPSC repository5. Age, sex,...
By means of sequencing, timing, prioritizing, matching, clustering, and other such rationalizing arrangements, it may be possible to use a fixed stock of resources to achieve higher productivity than is possible otherwise. For instance, provided that traffic flow conditions are within certain ...
It consists of an improvement of a ribosome footprinting approach [48] by using deep-sequencing, which References and recommended reading Papers of particular interest, published within the period of review, have been highlighted as:References (58)...
These mutants showed constitu- tive activity and Y705 phosphorylation in the absence of cytokine signaling.11 Similar results have been obtained in large granular lymphocytic leukemia using whole genome sequencing. In this disease, 40% of patients express a mutated form o...
4Formulator is finding the words and phrases to express the meanings, sequencing them and putting in appropriate grammatical markers.Articulation is the third process, it involves the motor control of the articulatory organs, in English: the lips, tongue, teeth, a 9、lveolar plate, velum, ...
least dimers are being formed. For footprinting reactions, the protein-DNA complexes, initially visualized in the gel retardation assays shown in Figure3A, were formed in solution and subjected to cleavage reactions at room temperature (20°C) prior to fractionation on 8% polyacrylamide sequencing ...
Single-cell messenger RNA sequencing reveals rare intestinal cell types. Nature 525, 251–255 (2015). Article ADS PubMed CAS Google Scholar Schuijers, J. et al. Ascl2 acts as an R-spondin/WNT-responsive switch to control stemness in intestinal crypts. Cell Stem Cell 16, 158–170 (...
Through whole exome sequencing of 92,445 participants in the MyCode cohort as part of the Geisinger-Regeneron DiscovEHR project, we found five participants heterozygous for early termination variants in the C-terminus of MC4R: three at Glutamine 307 (Q307Ter) (c.919 C > T) and two...