Hi, First, thanks for making STAR. It is a great aligner that has made it much easier for me to setup an RNA-seq workflow! I recently analysed a 15-sample RNA-seq, and one of the samples has around 80 % in % of reads unmapped: too short ...
The mean read length was B135 bp. On average, for every sample, 99% of high-quality sequencing reads (98% of bases) mapped to the reference genome. This resulted in an evenly distributed mean sequencing depth for NF1 and SPRED1 of 400X. On average, 98.5% of all targeted bases were ...
A parallel file system reads and writes data to distributed storage devices using multiple I/O paths concurrently, as part of one or more processes of a computer program. The coordinated use of multiple I/O paths can provide a significant performance benefit, especially when streaming workloads t...
The second example, which removes the the second set of dialogue tags, reads faster and is simpler. Simple is always better. If you are writing withmultiple (three or more) characters, use only enough dialogue tags to clearly indicate who is speaking. ...
As seen in the above examples, data egress charges can escalate quickly. This unconstrained cost model means potential misconfigurations, such as connecting to the wrong endpoint of a geo-replicated storage account, can lead to out-of-control cloud bills. So how does an IT team manage this ...
When you use Grammarly or ProWritingAid or any of the proofreading tools we’ve talked about, your writing may improve. But this does not mean you should skip hiring an editor before publishing. Every author needs to have their work professionallyedited by a qualified human editorbefore it is...
Sequencing of target-enriched DNA libraries were performed using an Illumina GAIIx, generating 75 bp single-end sequence reads. Image analysis and base calling was performed using the Genome Analyser Pipeline v1.5-1.7. Sequence reads were aligned to the human reference genome (GRCh37/hg19 assembly...
Does it capture screenshots or a video of script running, so you can see what the screen looked like when a button or form field couldn’t be found? Can you export the script in an industry-standard format so you can troubleshoot it somewhere else if need be?
What message is being written? Writing has been busy writing, but writing does not know what they are writing, because writing nothing to write, writing seems to write a lot, in fact, a word is not written, nothing is written out, simply white writing. ...
Illumina MiSeq paired-end sequencing of the AE59-TE2 library yielded 532,201 raw reads. After preprocessing steps, 365,719 (68.72%) quality reads were obtained (Supplementary Table S2). De novo genome assembly generated 109 contigs (Table 1) and the mapping genome assembly resulted in one sc...