Male reproductive system ageing is closely associated with deficiency in testosterone production due to loss of functional Leydig cells, which are differentiated from stem Leydig cells (SLCs). However, the relationship between SLC differentiation and age
Discusses the hypothesis that the individual WW domains in ubiquitin-protein ligase Rsp5p interact with different proteins and direct it to multiple targets. Contribution of individual WW domains to Rsp5p function; Differential effects of individual WW domain mutants on fluid phase endocytosis and intern...
Human campylobacteriosis usually presents as diarrhoea, however, clinical manifestations can also include (but are not limited to) long-term complications such as reactive arthritis and Guillain-Barré syndrome. The cost of campylobacteriosis in low and middle-income countries remains unknown, but it ...
- 《Current Biology》 被引量: 406发表: 2004年 Xenopus Neuralized Is a Ubiquitin Ligase that Interacts with XDelta1 and Regulates Notch Signaling Notch signaling in Drosophila requires a RING finger (RF) protein encoded by neuralized. Here we show that the Xenopus homolog of neuralized (Xneur...
the purified samples using the Blunt/TA Ligase Master Mix (NEB, M0367L). When using the SQK-LSK308 kit, two adapter ligation steps were required. First, the 1D2 adapter was ligated to the purified samples using the Blunt/TA Ligase Master Mix. After a 0.4X purification with Agencourt AM...
Preliminary results from this analysis identify an association between the RNA exosome and a large complex of tRNA ligase enzymes, which could link defects in tRNA maturation to disease pathology. We are extending these studies to explore the possibility of tissue-specific cofactors by immuno...
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T4 RNA Ligase 1 (ssRNA Ligase) NEB Cat # M0204 AffinityScript Multi-Temp RT Agilent Cat # 600109 Kapa HiFi HotStart PCR ReadyMix Kapa Biosystems Cat # KK2601 Whole Skin Dissociation Kit, human Miltenyi biotec Cat # 130-101-540 Gentlemacs dissociator Miltenyi biotec Cat # 130-093-235 Gentlem...
PAL, phenylalanine ammonia-lyase; C4H, cinnamate-4-hydroxylase; 4CL, 4-coumarate CoA ligase; CAD, cinnamoyl-alcohol dehydrogenase; F5H, ferulate 5-hydroxylase; C3H, coumarate 3-hydroxylase; ALDH, aldehyde dehydrogenase; CCR, cinnamoyl-CoA reductase; HCT, hydroxycinnamoyl-CoA shikimate/quinate ...
Gabriel, R., et al.Comprehensive genomic access to vector integration in clinical gene therapy.Nat Med.15(12), 1431-1436 (2009). Gillet, N. A., et al.The host genomic environment of the provirus determines the abundance of HTLV-1-infected T-cell clones.Blood.117(11), 3113-3122...