Define DNA ligase. DNA ligase synonyms, DNA ligase pronunciation, DNA ligase translation, English dictionary definition of DNA ligase. DNA A. adenine T. thymine C. cytosine G. guanine n. A nucleic acid that carries the genetic information in cells and so
Unit definition:Onecircularizationunit (or Modrich-Lehman unit) is the amount ofenzymewhich converts linear poly[d(AT)] (optimum length of 1000 nt) into 1 nmol of exonuclease Ill-resistant circular form in 30 min at 30°C. A slightly different type of ligase assay, developed by Olivera and...
for. Recombinant DNA associations form spontaneously in the above manner, but these associations are not stable because, although the ends are paired, the sugar-phosphatebackboneof the DNA has not been sealed. This is accomplished by the application of an enzyme called DNAligase, which seals the...
DNA ligase 1 finalizes nuclear DNA replication by accurately sealing nicks generated during Okazaki Fragment Maturation. Here, Williams et al employ a low fidelity DNA ligase 1 variant to study mutation rates and specificity across the yeast genome ...
Definition Chapters and Articles Related Terms Recommended Publications Featured Authors Chapters and Articles You might find these chapters and articles relevant to this topic. Cloning Genes for Analysis David P.Clark,Nanette J.Pazdernik, inMolecular Biology (Second Edition), 2013 ...
Reduced levels of Arabidopsis DNA ligase 1 in the silenced lines are sufficient to support plant development but result in retarded growth and reduced cell size, which may reflect roles for AtLIG1 in both replication and repair. The finding that DNA ligase 1 plays an important role in DSB rep...
R. Intramitochondrial recruitment of endolysosomes mediates Smac degradation and constitutes a novel intrinsic apoptosis antagonizing function of XIAP E3 ligase. Cell Death Differ. 21, 1862–1876 (2014). Article CAS PubMed PubMed Central Google Scholar Day, R. A. & Sletten, E. M. Experimental...
MBI EL0015 T4 DNA Ligase, LC MBI EL0016 T4 DNA Ligase, LC MBI EL0017 T4 DNA Ligase, HC MBI EL0021 T4 RNA Ligase MBI EL0331 T4 DNA Ligase (with PEG) MBI EL0334 T4 DNA Ligase (with PEG) MBI EL0335 T4 DNA Ligase, LC (with PEG) ...
Unit Definition:0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes. Protocols Complete Protocol T4 DNA Ligase, Blue/White Cloning Qualified ProtocolPDF(112 KB)– English ...
TASUKU HONJO, in Molecular Biology of B Cells, 2004 DNA Ligase IV The protein complex DNA ligase IV/XRCC4 is essential for NHEJ and for V(D)J recombination (Gellert, 2002). A recent report indicates that DNA ligase IV may also be involved in CSR, because a human patient with a mutant...