The density of protein in supernatants or tissue homogenates were evaluated by Micro BCA Protein Assay kits (Pierce, Rockford, IL); moreover, the counterpart was supplemented on a 96-well plate (coated with designative antibodies). The analysis of NGF density was consistent with the instruction ...
The present study demonstrated that JPH3 is the major pancreatic isoform, which expressed in human and mouse pancreatic beta cells. In mouse islets,Jph3deficiency caused the acute damage of GSIS, which was associated with the impairment of ER–mitochondria axis integrity. Therefore, our finding rev...
which contributes to the differentiation of ES cells into INS+cells and insulin secretion. These results highlight the crucial aspects of PPARβ/δ/Foxo1 on the generation of functional INS+cells. Therefore, the data may provide new insights into the underlying mechanisms by which PPARβ...