anti-Bcl-XL mAb, anti-Bax mAb and anti-β-actin mAb (Cell Signaling Technology, Danvers, MA, USA) and then visualized with an HRP-conjugated secondary antibody (Southern Biotech, UAB, Birmingham, AL, USA) and chemilumiescence (Thermo Pierce, Rockford, IL, USA) to further detect apoptosis...
rehydrated through an ethanol gradient, and then treated with 20 μg/mL proteinase K (Roche Diagnostics, Indianapolis, IN, USA). Then, it was fixed with formaldehyde (Thermo Scientific, Rockford, IL, USA), rinsed twice with 0.13 M 1-methylimidazole...
Silver staining: the gel was stained with the Pierce Silver Stain Kit (Thermo Scientific, Rockford, IL) according to the manufacturer’s instructions. Preparation and culture of human NK cells Human NK cells were purified out of leukocyte concentrates from the blood bank in Mannheim (Germany). ...
Final bands were visualized by ECL kit (Pierce Biotechnology, Rockford, IL, USA). Image J was applied to quantify the intensity of the band. Experiments were performed in triplicate. Plasmid transfection The full-length wild-type human SNHG1 and 401-607 bp truncated mutant SNHG1-mut were...
We present here a new, classification-based screening method for anti-cancer botanical combinations. Using this method, we discovered that the combination of Astragalus membranaceus and Vaccaria hispanica (AV) has strong synergic anti-proliferative and k