rehydrated through an ethanol gradient, and then treated with 20 μg/mL proteinase K (Roche Diagnostics, Indianapolis, IN, USA). Then, it was fixed with formaldehyde (Thermo Scientific, Rockford, IL, USA), rinsed twice with 0.13 M 1-methylimidazole...
Final bands were visualized by ECL kit (Pierce Biotechnology, Rockford, IL, USA). Image J was applied to quantify the intensity of the band. Experiments were performed in triplicate. Plasmid transfection The full-length wild-type human SNHG1 and 401-607 bp truncated mutant SNHG1-mut were...