rehydrated through an ethanol gradient, and then treated with 20 μg/mL proteinase K (Roche Diagnostics, Indianapolis, IN, USA). Then, it was fixed with formaldehyde (Thermo Scientific, Rockford, IL, USA), rinsed twice with 0.13 M 1-methylimidazole...
We present here a new, classification-based screening method for anti-cancer botanical combinations. Using this method, we discovered that the combination of Astragalus membranaceus and Vaccaria hispanica (AV) has strong synergic anti-proliferative and k