Christopher M TrainiMin WangUSUS5989858 * 1997年10月28日 1999年11月23日 Smithkline Beecham Corporation Dead-type ATP-dependent RNA helicase (DbpB) from Staphylococcus aureusUS5989858 * Oct 28, 1997 Nov 23, 1999 Smithkline Beecham Corporation Dead-type ATP-dependent RNA helicase (DbpB) from ...
box proteins for RNA. Thus, the ATP hydrolysis is necessary for the release of DEAD-box helicase from the RNA substrate, and promotes substrate turnover[78]. In contrast with other processive helicases (for example, Pif1) which translocate along DNA or RNA substrate during ATP hydrolysis ...
Despite high conservation between DEAD box proteins, they participate in many different processes. Recent structural and functional analyses have changed our perception of these fascinating enzymes. They clamp the RNA substrate in an ATP-dependent manner, which can lead to the formation of an RNA-...
DDX3 is a highly conserved member of ATP-dependent DEAD-box RNA helicases with multiple functions in RNA metabolism and cellular signaling. Here, we describe a novel function for DDX3 in regulating the mitochondrial stress response in the parasitic protozoanLeishmania.We show that genetic inactivatio...
Keywords:RNAhelicase;DEAD boxRNAhelicase;neoplasm 1 引言 RNA解旋酶有 9个保守基序,其中含有能够结 合 RNA和 NTP的结构域.RNA解旋酶与 RNA结 合后可以激活 NTP酶的活性,水解 NTP产生能量促 使双链 RNA解链.DEAD boxRNA解旋酶(DEAD boxRNAHelicase,DDX)是 RNA解旋酶超家族成员 之一,因为其第二个基序含有...
DEAD-box proteins catalyze the ATP-dependent unwinding of RNA duplexes. The common unit of these enzymes is a helicase core of two flexibly linked RecA domains. ATP binding and phosphate release control opening and closing of the cleft in the helicase core. This movement coordinates RNA-binding...
DEAD-box proteins, the largest helicase family, catalyze ATP-dependent remodeling of RNA-protein complexes and the unwinding of RNA duplexes. Because DEAD-... L Fei,A Putnam,E Jankowsky - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 382发表: ...
thaliana DEAD-box ATP-dependent RNA helicase 7 (also known as AtRH7; AT5G62190) was previously shown experimentally to be localized to the nucleus (Lorković et al. 1997). This evidence supports our phylogenetic analysis suggesting that GUCT-containing RNA helicases are clearly organelle ...
transcriptional level. RNA helicases are involved in the formation of cytoplasmic ribonucleoprotein (RNP) granules and RNA silencing. A highly conserved central domain is characteristic of DEAD-box RNA helicases and determines their basic biological activity in ATP-dependent unwinding of short RNA ...
Here we show that members of the large and highly abundant family of RNA-dependent DEAD-box ATPases (DDXs)3 are regulators of RNA-containing phase-separated organelles in prokaryotes and eukaryotes. Using in vitro reconstitution and in vivo experiments, we demonstrate that DDXs promote phase ...