2. Coverslips containing cells were placed in 5 µM H2DCFDA staining solution and incubated for 60 min at 37°C, protected from light, then washed and imaged with a confocal laser scanning microscope Leica TCS SL equipped with an argon laser. [1] In vivo METHODS: Fluorescence microscopy ...
3. Along with the H2DCFDA probe, if indicated, use ROS-insensitive modification of the fluorescein dye DCFDA as a positive control. The staining procedure is the same as for the H2DCFDA。 Emission (Em)=517 Excitation (Ex)=492 517