METHODS: Fluorescence microscopy was used to analyze the oxidative activity of LPS induced peritonitis in mice: 1, H2DCFDA was dissolved in 100 µL ethanol and further diluted with PBS before use. 2. C57BL/6J mice were injected intraperitoneally with LPS (0.1-1 mg/mL) to induce peritonitis...
three times for each treatment. Mean fluorescence intensity is quantified using software. Cells that had been incubated without DCFH-DA are used as negative control. The distribution of fluorescent dichlorofluorescein (DCF) on the cell monolayer is visualized and photographed using a fluorescence ...
Resuspend cells in PBS, seed in 96-well microplate with 100,000 stained cells/well and measure fluorescence immediately. *X represents the volume of DCFH-DA to obtain the optimal concentration related to the cell line used. NOTE: To create positive controls, oxidative activity is stimulated with...