2. Coverslips containing cells were placed in 5 µM H2DCFDA staining solution and incubated for 60 min at 37°C, protected from light, then washed and imaged with a confocal laser scanning microscope Leica TCS SL equipped with an argon laser. [1] ...
Once the incubation is completed, DO NOT wash the cells After staining, treat the cells with compound(s) of interest and ensure that appropriate 4 controls are included. If using THBP as positive control, optimal signal is obtained after 4 hours of treatment. Analyze on flow cytometer. ...
CaspGLOW Fluorescein Active Caspase-3 Staining Kit HEPES (1 M) LX-2人肝星形细胞 SGC7901SGC7901 Total Exosome Isolation Reagent (from urine) PLASMOCIN TREATMENT Mycoplasma EliminatorPlasmocin? treatment支原体清除剂 Ultrafiltration tube [4ML 3KD], detachable超滤管[4ml 3KD],可拆开 ...
3. Along with the H2DCFDA probe, if indicated, use ROS-insensitive modification of the fluorescein dye DCFDA as a positive control. The staining procedure is the same as for the H2DCFDA。 Emission (Em)=517 Excitation (Ex)=492 517